Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits
文献类型：期刊论文
| 作者 | Zhao, Xue1; Han, Feng2; Shen, Shihua
|
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2014 |
| 卷号 | 59期号:13页码:1392-1404 |
| 关键词 | Plastid differentiation High pigment-1 Proteomics Tomato fruit ripening Two-dimensional electrophoresis (2-DE) |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-014-0141-9 |
| 文献子类 | Article |
| 英文摘要 | The chloroplast-to-chromoplast difference is a highly regulated process. Tomato (Solanum lycopersicum) high-pigment mutant hp-1 shows an enhanced accumulation of carotenoids coupled with an increase in the volume and number of plastids. However, how chromoplasts of hp-1 acquire their specific metabolic characteristics is still unclear. A comparison of proteome profiles from plastids at the mature green, breaker, and red stages of tomato fruits showed 45 differentially expressed proteins. These identified proteins fell into six different functional categories. Our results showed that most of the proteins related to the Calvin cycle increased transitorily only at the early breaker stage, and remained unchanged at the early red stage. We found 18 proteins that were differentially expressed between the wild type and hp-1. The abundance of GCPE in chromoplasts of hp-1 was higher than that in the wild type. Meanwhile, we found that heat shock proteins were only present specifically in the mature green stage chromoplasts of hp-1 but not in the wild type. This suggested that GCPE and heat shock proteins might play important roles in the accumulation of high carotenoids in hp-1. Overall, our results could be helpful for understanding chloroplast-to-chromoplast differentiation and the function of hp-1. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | BEIJING |
| 电子版国际标准刊号 | 1861-9541 |
| WOS关键词 | CAROTENOID BIOSYNTHESIS ; CHROMOPLAST TRANSITION ; FTSH PROTEASE ; CHLOROPLAST ; SYNTHASE ; PROTEINS ; PATHWAYS ; REVEALS ; PLANTS ; STRESS |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000333613100007 |
| 出版者 | SCIENCE PRESS |
| 资助机构 | Key Directional Project from the Chinese Academy of Sciences [KZCX2-YW-359-2] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27075]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xue,Han, Feng,Shen, Shihua. Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits[J]. CHINESE SCIENCE BULLETIN,2014,59(13):1392-1404. |
| APA | Zhao, Xue,Han, Feng,&Shen, Shihua.(2014).Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits.CHINESE SCIENCE BULLETIN,59(13),1392-1404. |
| MLA | Zhao, Xue,et al."Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits".CHINESE SCIENCE BULLETIN 59.13(2014):1392-1404. |
入库方式： OAI收割
来源：植物研究所
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