Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera
文献类型：期刊论文
| 作者 | Sun, Jingwen1; Peng, Xianjun; Fan, Weihong; Tang, Mingjuan; Liu, Jie; Shen, Shihua
|
| 刊名 | GENE
 |
| 出版日期 | 2014 |
| 卷号 | 535期号:2页码:140-149 |
| 关键词 | AP2/EREBP-domain Broussonetia papyrifera DREB Salt and cold tolerance Woody plant |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2013.11.047 |
| 文献子类 | Article |
| 英文摘要 | The dehydration-responsive element binding proteins (DREBs) are important transcription factors in the regulation of plant responses to abiotic stresses. In this study, BpDREB2, an AP2/DREB-type transcription factor gene, was cloned from a woody plant, Broussonetia papyrifera by RACE-PCR Sequence analyses revealed that BpDREB2 protein has three characteristic domains, including an AP2/EREBP, a nuclear localization signal and an acidic activation domain. Yeast one-hybrid assays showed that BpDREB2 protein specifically binds to the ORE sequence and activates the expression of reporter genes in yeast. These results suggested that BpDREB2 protein could function as a transcription factor of DREB family. The expression of BpDREB2 gene was remarkably induced by dehydration and high-salt treatments, but no significant change was observed under ABA or low-temperature conditions. Importantly, transgenic expression of BpDREB2 gene in Arabidopsis significantly enhanced its tolerance to salt and freezing without causing growth retardation. Taken together, these results suggested that BpDREB2 is a novel member of the AP2/EREBP trans-acting factor family which could enhance salt stress tolerance of plants and has the potential application in the improvement of crops and economical tree species. (C) 2013 Elsevier B.V. All rights reserved. |
| 学科主题 | Genetics & Heredity |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1879-0038 |
| WOS关键词 | BINDING TRANSCRIPTION FACTOR ; FREEZING TOLERANCE ; ARABIDOPSIS ; COLD ; DEHYDRATION ; PROTEINS ; ELEMENT ; EXPRESSION ; L. ; STRESSES |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000331672200009 |
| 出版者 | ELSEVIER |
| 资助机构 | Chinese Academy of Sciences [KSCX2-YW-G-359-2] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27077]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Crop Nutr & Fertilizat, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jingwen,Peng, Xianjun,Fan, Weihong,et al. Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera[J]. GENE,2014,535(2):140-149. |
| APA | Sun, Jingwen,Peng, Xianjun,Fan, Weihong,Tang, Mingjuan,Liu, Jie,&Shen, Shihua.(2014).Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera.GENE,535(2),140-149. |
| MLA | Sun, Jingwen,et al."Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera".GENE 535.2(2014):140-149. |
入库方式： OAI收割
来源：植物研究所
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