LcWRKY5: an unknown function gene from sheepgrass improves drought tolerance in transgenic Arabidopsis
文献类型：期刊论文
| 作者 | Ma, Tian1; Li, Manli2; Zhao, Aiguo; Xu, Xing1; Liu, Gongshe ; Cheng, Liqin
|
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 33期号:9页码:1507-1518 |
| 关键词 | Sheepgrass Transcriptional factor LcWRKY5 Drought |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-014-1634-3 |
| 文献子类 | Article |
| 英文摘要 | The expression of LcWRKY5 was induced significantly by salinity, mannitol and cutting treatments. Arabidopsis- overexpressing LcWRKY5 greatly increased dehydration tolerance by regulating the expression of multiple stress-responsive genes. Based on the data of sheepgrass 454 high-throughout sequencing and expression analysis results, a drought-induced gene LcWRKY5 was isolated and cloned, and the biological role of the gene has not been reported until now. Bioinformatics analysis showed that LcWRKY5 contains one conserved WD domain and belongs to the group II WRKY protein family. LcWRKY5 shows high sequence identity with predicted or putative protein products of Hordeum vulgare, Aegilops tauschii, Triticum aestivum, Brachypodium distachyon, Oryza sativa, but it has low homology with WRKYs from dicotyledonous plants. Several drought-inducibility, fungal elicitor, MeJA-responsiveness, endosperm, light, anoxic specific inducibility, and circadian control elements were found in the promoter region of LcWRKY5. Tissue-specific expression patterns showed that LcWRKY5 is expressed in roots and leaves, without expression in other tissues. The expression of LcWRKY5 was induced significantly under salinity and mannitol stresses but was not notably changed under cold and Abscisic acid stress. The LcWRKY5 protein exhibits transcription activation activity in the yeast one-hybrid system. Overexpressing LcWRKY5 exhibited increased rates of cotyledon greening and plant survival in transgenic Arabidopsis compared with wild-type plants under drought stress, and the expression levels of DREB2A and RD29A in transgenic plants were enhanced under drought stress. These results indicated that LcWRKY5 may play an important role in drought-response networks through regulation of the DREB2A pathway. LcWRKY5 can be a candidate gene for engineering drought tolerance in other crops. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-203X |
| WOS关键词 | WRKY TRANSCRIPTION FACTOR ; DNA-BINDING ; EXPRESSION ANALYSIS ; ABIOTIC STRESS ; PLANT DEFENSE ; FACTOR FAMILY ; SUPERFAMILY ; ACCUMULATION ; NETWORKS ; PATHOGEN |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000340590900008 |
| 出版者 | SPRINGER |
| 资助机构 | National Basic Research Program of China (973'') [2014CB138704] ; Ningxia 3 Agricultural Comprehensive Development Office [NTKJ-2013-03(1)] ; National Natural Science Foundation of China [31170316] ; National High Technology Research and Development Program of China (863'') [2011AA100209] ; Ministry of Agriculture of China [2009ZX08009-097B] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27082]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Ning Xia Univ, Coll Agr, Ningxia 750021, Peoples R China 3.China Agr Univ, Dept Grassland Sci, Coll Anim Sci & Technol, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Tian,Li, Manli,Zhao, Aiguo,et al. LcWRKY5: an unknown function gene from sheepgrass improves drought tolerance in transgenic Arabidopsis[J]. PLANT CELL REPORTS,2014,33(9):1507-1518. |
| APA | Ma, Tian,Li, Manli,Zhao, Aiguo,Xu, Xing,Liu, Gongshe,&Cheng, Liqin.(2014).LcWRKY5: an unknown function gene from sheepgrass improves drought tolerance in transgenic Arabidopsis.PLANT CELL REPORTS,33(9),1507-1518. |
| MLA | Ma, Tian,et al."LcWRKY5: an unknown function gene from sheepgrass improves drought tolerance in transgenic Arabidopsis".PLANT CELL REPORTS 33.9(2014):1507-1518. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。