Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis
文献类型：期刊论文
| 作者 | Yu, Pengli; Yuan, Jinhong; Deng, Xin1,2 ; Ma, Mi; Zhang, Haiyan
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| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 55期号:9页码:1568-1581 |
| 关键词 | Arabidopsis Cu accumulation CusF Phytoremediation Subcellular targeting Translocation factor |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcu087 |
| 文献子类 | Article |
| 英文摘要 | Copper (Cu) is an important environmental pollutant that exerts harmful effects on all living organisms when in excess. In an effort to remove this toxin in situ, a bacterial Cu-binding protein gene CusF was engineered to target CusF for secretion to the cell wall and vacuoles and for accumulation in the cytoplasm. Analysis of transgenic Arabidopsis plants showed that CusF was functionally active and that plants expressing cell wall-(CusF(cw) transgenic lines) or vacuole-targeted CusF (CusF(vac) transgenic lines) were more resistant to Cu excess than untransformed plants and plants with cytoplasmic CusF (CusF(cyto) transgenic lines). Under short-term (48 h) exposure to Cu excess, CusFcw transgenic lines showed up to 2-fold increased Cu accumulation in roots compared with the untransformed plants; however, CusFcyto lines and the wild-type plants had similar Cu concentrations in both roots and shoots. Under long-term (40 d) exposure to Cu excess, all transgenic lines accumulated more Cu (up to 3-fold) in roots than the untransformed plants, whereas only CusFcyto lines showed a marked increase (similar to 3-fold of the wild-type plants) of Cu accumulation in shoots. In addition, expression of CusF in the cytosol dramatically enhanced Cu transport from roots to shoots when compared with plants with secretory pathway-targeted CusF. Our results demonstrate the feasibility of Cu tolerance and accumulation by engineering Cu-binding proteins targetable to subcellular compartments and provide new insights into the multifaceted mechanisms of Cu partitioning between roots and shoots. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1471-9053 |
| WOS关键词 | ESCHERICHIA-COLI ; COPPER TRANSPORT ; ENDOPLASMIC-RETICULUM ; IRON HOMEOSTASIS ; CELL-WALL ; PH ; METALLOTHIONEINS ; COMPARTMENTATION ; TOLERANCE ; CADMIUM |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000342978300005 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China [31270313, 90817010] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27087]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Yu, Pengli,Yuan, Jinhong,Deng, Xin,et al. Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis[J]. PLANT AND CELL PHYSIOLOGY,2014,55(9):1568-1581. |
| APA | Yu, Pengli,Yuan, Jinhong,Deng, Xin,Ma, Mi,&Zhang, Haiyan.(2014).Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis.PLANT AND CELL PHYSIOLOGY,55(9),1568-1581. |
| MLA | Yu, Pengli,et al."Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis".PLANT AND CELL PHYSIOLOGY 55.9(2014):1568-1581. |
入库方式： OAI收割
来源：植物研究所
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