Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars
文献类型：期刊论文
| 作者 | Fang, Linchuan1; Hou, Yanlin2; Wang, Lijun3; Xin, Haiping3; Wang, Nian4; Li, Shaohua3
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| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 33期号:10页码:1629-1640 |
| 关键词 | Resveratrol Stilbene synthases STS Myb14 Grapevine |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-014-1642-3 |
| 文献子类 | Article |
| 英文摘要 | High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabolism in some plants. Stilbene synthase (STS) is the key enzyme for the final step of precursor formation of resveratrol (Res) in grapevines. In this study, we found that Res contents in ripe berry skin were completely different in two grape cultivars, namely, 'Z168' (Vitis monticola x Vitis riparia) with high-Res and 'Jingzaojing' (Vitis vinifera) with low-Res. Moreover, the level of expression of STS gene was higher in the ripe berry skin of 'Z168' than in that of 'Jingzaojing'. To further investigate the underlying mechanisms, we conducted a co-expression analysis through transcriptomic data. We confirmed that Myb14, an R2R3 Myb transcription factor, is the direct regulator of STS by binding to Box-L5 motif. Moreover, the expression pattern of Myb14 is associated with the variation of Res content. To test this prediction, we conducted a number of experiments in vivo and in vitro. The expression patterns of Myb14 and STS in grapevine leaves were identical under a series of stimulus. Myb14 showed higher expression in the ripe berry skin of 'Z168' than in that of 'Jingzaojing'. Yeast one-hybrid assay indicated that grapevine Myb14 could interact with the promoter of STS in vitro, and the transient overexpression of Myb14 promoted the expression of STS. Furthermore, co-expressing 35S::Myb14 in transgenic Arabidopsis could activate GUS expression promoted by STS promoter. Thus, Myb14 is the direct activator of STS, and its expression pattern is associated with Res content variation in grapes. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-203X |
| WOS关键词 | PHENYLALANINE AMMONIA-LYASE ; STILBENE SYNTHASE ; GENE-EXPRESSION ; VITIS-VINIFERA ; CABERNET-SAUVIGNON ; MOLECULAR ANALYSIS ; CHALCONE SYNTHASE ; UV-C ; BIOSYNTHESIS ; TOLERANCE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000342193100003 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China (NSFC) [31171931] ; National Natural Science Foundation of Hubei Province [2011CDB409] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27092]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Beijing, Peoples R China 5.Chinese Acad Agr Sci, Oil Crop Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Linchuan,Hou, Yanlin,Wang, Lijun,et al. Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars[J]. PLANT CELL REPORTS,2014,33(10):1629-1640. |
| APA | Fang, Linchuan,Hou, Yanlin,Wang, Lijun,Xin, Haiping,Wang, Nian,&Li, Shaohua.(2014).Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars.PLANT CELL REPORTS,33(10),1629-1640. |
| MLA | Fang, Linchuan,et al."Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars".PLANT CELL REPORTS 33.10(2014):1629-1640. |
入库方式： OAI收割
来源：植物研究所
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