Proteome analysis for antifungal effects of Bacillus subtilis KB-1122 on Magnaporthe grisea P131
文献类型：期刊论文
| 作者 | Zhang, Caixia1,2; Zhang, Xinxiong3; Shen, Shihua1
|
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 30期号:6页码:1763-1774 |
| 关键词 | Bacillus subtilis Antifungal effects Co-culture Magnaporthe grisea Proteomics |
| ISSN号 | 0959-3993 |
| DOI | 10.1007/s11274-014-1596-1 |
| 文献子类 | Article |
| 英文摘要 | Rice blast, caused by Magnaporthe grisea threatens rice production worldwide. It is important to develop novel and environment-safe strategies to control the fungus. Here we reported that Bacillus subtilis KB-1122 could strikingly inhibit the growth of M. grisea P131 in agar diffusion assays. To further understand the molecular mechanism on the suppressive role of B. subtilis on M. grisea, the antagonist-pathogen interaction of the two strains was studied by using comparative proteome analysis in this report. The cellular and culture supernatant (CSN) proteins were prepared from co-culture and subjected to two-dimensional polyacrylamide gel electrophoresis. Proteome analysis revealed 33 cellular and 18 CSN proteins showing changes upon co-culture respectively. Importantly, down-regulated cellular proteins came from M. grisea, whereas up-regulated proteins derived from B. subtilis. Results suggested that glyceraldehyde-3-phosphate dehydrogenase and serine protein kinase might contribute to antifungal activity of B. subtilis KB-1122. Of CSN proteins identified, the endo-1,4-beta-glucanase (involved in degradation of polysaccharides) was up-regulated consistently at different times of incubation. This suggests that this enzyme plays an important role in the interaction between B. subtilis KB-1122 with M. grisea P131. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1573-0972 |
| WOS关键词 | POSTHARVEST BIOLOGICAL-CONTROL ; RICE LEAF SHEATH ; TRICHODERMA-HARZIANUM ; PROTEINS ; BIOCONTROL ; STRAINS ; IDENTIFICATION ; DISEASES ; EXPRESSION ; INFECTION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000338136800009 |
| 出版者 | SPRINGER |
| 资助机构 | State Key Basic Research and Development Plan of China [2010CB126503] ; China Agriculture Research System [CARS-28] ; National Natural Science Foundation of China [30900968] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27101]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Agr Sci, Res Inst Pomol, Xingcheng 125100, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops Germp, Xingcheng 125100, Liaoning, Peoples R China 4.Dongguan Baode Biol Engn Co Ltd, Dongguan 523087, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Caixia,Zhang, Xinxiong,Shen, Shihua. Proteome analysis for antifungal effects of Bacillus subtilis KB-1122 on Magnaporthe grisea P131[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2014,30(6):1763-1774. |
| APA | Zhang, Caixia,Zhang, Xinxiong,&Shen, Shihua.(2014).Proteome analysis for antifungal effects of Bacillus subtilis KB-1122 on Magnaporthe grisea P131.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,30(6),1763-1774. |
| MLA | Zhang, Caixia,et al."Proteome analysis for antifungal effects of Bacillus subtilis KB-1122 on Magnaporthe grisea P131".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 30.6(2014):1763-1774. |
入库方式： OAI收割
来源：植物研究所
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