Multiple loss-of-function 5-O-glucosyltransferase alleles revealed in Vitis vinifera, but not in other Vitis species
文献类型：期刊论文
| 作者 | Yang, Yingzhen; Labate, Joanne A.; Liang, Zhenchang2; Cousins, Peter; Prins, Bernard; Preece, John E.; Aradhya, Mallikarjuna3; Zhong, Gan-Yuan |
| 刊名 | THEORETICAL AND APPLIED GENETICS
 |
| 出版日期 | 2014 |
| 卷号 | 127期号:11页码:2433-2451 |
| ISSN号 | 0040-5752 |
| DOI | 10.1007/s00122-014-2388-6 |
| 文献子类 | Article |
| 英文摘要 | Wild and loss-of-function alleles of the 5 - O - glucosyltransferase gene responsible for synthesis of diglucoside anthocyanins in Vitis were characterized. The information aids marker development for tracking this gene in grape breeding. Anthocyanins in red grapes are present in two glycosylation states: monoglucoside (3-O-glucoside) and diglucoside (3, 5-di-O-glucoside). While monoglucoside anthocyanins are present in all pigmented grapes, diglucoside anthocyanins are rarely found in the cultivated grape species Vitis vinifera. Biochemically 3-O-glucoside anthocyanins can be converted into 3,5-di-O-glucoside anthocyanins by a 5-O-glucosyltransferase. In this study, we surveyed allelic variation of the 5-O-glucosyltransferase gene (5GT) in 70 V. vinifera ssp. vinifera cultivars, 52 V. vinifera ssp. sylvestris accessions, 23 Vitis hybrid grapes, and 22 accessions of seven other Vitis species. Eighteen 5GT alleles with apparent loss-of-function mutations, including seven premature stop codon mutations and six frameshift indel mutations, were discovered in V. vinifera, but not in the other Vitis species. A total of 36 5GT alleles without apparent loss-of-function mutations (W-type) were identified. These W-type alleles were predominantly present in wild Vitis species, although a few of them were also found in some V. vinifera accessions. We further evaluated some of these 5GT alleles in producing diglucoside anthocyanins by analyzing the content of diglucoside anthocyanins in a set of representative V. vinifera cultivars. Through haplotype network analysis we revealed that V. vinifera ssp. vinifera and its wild progenitor V. vinifera ssp. sylvestris shared many loss-of-function 5GT alleles and extensive divergence of the 5GT alleles was evident within V. vinifera. This work advances our understanding of the genetic diversity of 5GT and provides a molecular basis for future marker-assisted selection for improving this important wine quality trait. |
| 学科主题 | Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2242 |
| WOS关键词 | COLOR STABILITY ; ANTIOXIDANT CAPACITY ; MOLECULAR-CLONING ; GRAPE CULTIVARS ; RIPE BERRIES ; RED WINE ; ANTHOCYANINS ; ROTUNDIFOLIA ; 3,5-DIGLUCOSIDE ; GENE |
| WOS研究方向 | Agriculture ; Plant Sciences ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000344343500011 |
| 出版者 | SPRINGER |
| 资助机构 | United States Department of Agriculture, Agricultural Research Service, CRIS Project [1910-21000-020-00D] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27137]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA 2.USDA ARS, Plant Genet Resources Unit, Geneva, NY 14456 USA 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.UCD, USDA Natl Clonal Germplasm Repository, Davis, CA 95616 USA |
| 推荐引用方式 GB/T 7714 | Yang, Yingzhen,Labate, Joanne A.,Liang, Zhenchang,et al. Multiple loss-of-function 5-O-glucosyltransferase alleles revealed in Vitis vinifera, but not in other Vitis species[J]. THEORETICAL AND APPLIED GENETICS,2014,127(11):2433-2451. |
| APA | Yang, Yingzhen.,Labate, Joanne A..,Liang, Zhenchang.,Cousins, Peter.,Prins, Bernard.,...&Zhong, Gan-Yuan.(2014).Multiple loss-of-function 5-O-glucosyltransferase alleles revealed in Vitis vinifera, but not in other Vitis species.THEORETICAL AND APPLIED GENETICS,127(11),2433-2451. |
| MLA | Yang, Yingzhen,et al."Multiple loss-of-function 5-O-glucosyltransferase alleles revealed in Vitis vinifera, but not in other Vitis species".THEORETICAL AND APPLIED GENETICS 127.11(2014):2433-2451. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。