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Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony

文献类型：期刊论文

作者Hao, Hai-ping; He, Zhi; Li, Hui; Shi, Lei; Tang, Yu-Dan
刊名ANNALS OF BOTANY
出版日期2014
卷号113期号:3页码:443-452
关键词Seed epicotyl dormancy release hormone root length Paeonia ludlowii Tibetan peony
ISSN号0305-7364
DOI10.1093/aob/mct273
文献子类Article
英文摘要Epicotyl dormancy break in seeds that have deep simple epicotyl morphophysiological dormancy (MPD) requires radicle emergence and even a certain root length in some species. However, the mechanisms by which root length affects epicotyl dormancy break are not clear at present. This study aims to explore the relationship between root length and epicotyl dormancy release in radicle-emerged seeds of Tibetan peony, Paeonia ludlowii, with discussion of the possible mechanisms. Radicle-emerged seeds (radicle length 15, 30, 45 and 60 cm) were incubated at 5, 10 and 15 C. During the stratification, some seeds were transferred to 15 C and monitored for epicotylplumule growth. Hormone content was determined by ELISA, and the role of hormones in epicotyl dormancy release was tested by exogenous hormone and embryo culture. Cold stratification did not break the epicotyl dormancy until the root length was 6 cm. The indole-3-actic acid (IAA) and GA(3) contents of seeds having 6 cm roots were significantly higher than those of seeds with other root lengths, but the abscisic acid (ABA) content was lowest among radicle-emerged seeds. GA(3) (400 mg L-1) could break epicotyl dormancy of all radicle-emerged seeds, while IAA (200 mg L-1) had little or no effect. When grown on MS medium, radicles of naked embryos grew and cotyledons turned green, but epicotyls did not elongate. Naked embryos developed into seedlings on a mixed medium of MS 100 mg L-1 GA(3). A root length of 60 cm is necessary for epicotyl dormancy release by cold stratification. The underlying reason for root length affecting epicotyl dormancy release is a difference in the GA(3)/ABA ratio in the epicotyl within radicle-emerged seeds, which is mainly as a result of a difference in ABA accumulation before cold stratification.
学科主题Plant Sciences
出版地OXFORD
电子版国际标准刊号1095-8290
WOS关键词ENDOGENOUS ABSCISIC-ACID ; ARABIDOPSIS-THALIANA ; GERMINATION ; MAINTENANCE ; DEFICIENT
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000331265200005
出版者OXFORD UNIV PRESS
资助机构National Key Technology RD Program [2012BAC01B05] ; Knowledge Innovation Engineering of the Chinese Academy of Sciences [KSCX2-EW-B-5] ; Strategic Technical Support System of Special Biological Resources Project [CZBZX-1]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27215]  
专题中科院北方资源植物重点实验室
作者单位1.[Hao, Hai-ping
2.He, Zhi
3.Li, Hui
4.Shi, Lei
5.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Hao, Hai-ping,He, Zhi,Li, Hui,et al. Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony[J]. ANNALS OF BOTANY,2014,113(3):443-452.
APA Hao, Hai-ping,He, Zhi,Li, Hui,Shi, Lei,&Tang, Yu-Dan.(2014).Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony.ANNALS OF BOTANY,113(3),443-452.
MLA Hao, Hai-ping,et al."Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony".ANNALS OF BOTANY 113.3(2014):443-452.

入库方式： OAI收割

来源：植物研究所

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