Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica
文献类型：期刊论文
| 作者 | Li, Yang; Shi, Lei
|
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 68期号:3页码:446-450 |
| 关键词 | Osmunda japonica Green spore Liquid nitrogen (LN) storage Desiccation Spore viability |
| ISSN号 | 0011-2240 |
| DOI | 10.1016/j.cryobiol.2014.03.002 |
| 文献子类 | Article |
| 英文摘要 | In order to effectively preserve green spores, which have relatively higher water content and lose viability more quickly than non-green spores, we studied the effect of desiccation level and storage temperature on Osmunda japonica spores. The water content of fresh spores was 11.20%. After 12 h desiccation by silica gel, the water content decreased to 6% but spore viability did not change significantly. As the desiccation continued, the decrease in water content slowed, but spore viability dropped. For almost all storage periods, the effects of storage temperature, desiccation level, and temperature x desiccation level were significantly different. After seven days of storage, spores at any desiccation level stored at 4 degrees C obtained high germination rates. After more than seven days storage, liquid nitrogen (LN) storage obtained the best results. Storage at 18 degrees C led to the lowest germination rates. Spores stored at room temperature and 18 degrees C all died within three months. For storage at 4 degrees C and in LN, spores desiccated 12 and 36 h obtained better results. Spores without desiccation had the highest germination rates after being stored at room temperature, but suffered the greatest loss after storage at 18 degrees C. These results suggest that LN storage is the best method of long-term storage of O. japonica spores. The critical water content of O. japonica spores is about 6% and reduction of the water content to this level improves outcome after LN storage greatly. The reason for various responses of O. japonica spores to desiccation and storage temperatures are discussed. (C) 2014 Elsevier Inc. All rights reserved. |
| 学科主题 | Biology ; Physiology |
| 出版地 | SAN DIEGO |
| 电子版国际标准刊号 | 1090-2392 |
| WOS关键词 | FERN SPORES ; EMBRYONIC AXES ; CRYOPRESERVATION ; SEEDS ; SURVIVAL ; WATER ; CONSERVATION ; GERMINATION ; HYPOTHESIS ; MOISTURE |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000336884100019 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | National Key Technology Support Program of the Ministry of Science and Technology of China [2012BAC01B05] ; Knowledge Innovative Program of The Chinese Academy of Sciences [KSCX2-EW-B-5] ; Special Program for Technology Supporting System of Strategic Biological Resources In Vitro Plant Germplasm Collection, BRC-CAS [CZBZX-1] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27217]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yang,Shi, Lei. Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica[J]. CRYOBIOLOGY,2014,68(3):446-450. |
| APA | Li, Yang,&Shi, Lei.(2014).Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica.CRYOBIOLOGY,68(3),446-450. |
| MLA | Li, Yang,et al."Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica".CRYOBIOLOGY 68.3(2014):446-450. |
入库方式： OAI收割
来源：植物研究所
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