The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.
文献类型：期刊论文
| 作者 | Shen, Hongling1; He, Zhenyan; Yan, Huili1; Xing, Zenan1; Chen, Yanshan1; Xu, Wenxiu; Xu, Wenzhong; Ma, Mi |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2014 |
| 卷号 | 105页码:46-57 |
| ISSN号 | 1874-3919 |
| 关键词 | Pteris vittata Vacuole transporter Compartmentalization Arsenic detoxification Membrane proteomic |
| DOI | 10.1016/j.jprot.2014.01.029 |
| 文献子类 | Article |
| 英文摘要 | Pteris vittata, the first known arsenic hyperaccumulating plant, can accumulate very high concentration arsenic in its aboveground tissues, while low in roots. Previous studies have suggested that arsenic vacuole compartmentalization may play an important role in the arsenic-hyperaccumulation in P. vittata, but the mechanism(s) of arsenic transport to vacuole are largely unknown. We obtained tonoplast isolated from fronds of P. vittata sporophyte grown under minus and 1 mM arsenate for 3 weeks by iodixanol step gradient centrifugation method, and then used TMPP protein labeling technology followed by liquid chromatography a linear ion trap-Orbitrap hybrid mass spectrometer analysis for the quantitative detection of proteins. And we designed and used an artificial database for database searching. In total, 56 tonoplast proteins were identified; more than 70% of them were transport proteins. Under arsenate treatment, one TDT transporter protein, a member of the TerC family and a PDR-like protein were upregulated differentially. While V-ATPase subunits c, E, and G, and V-PPase, were downregulated. Additionally, the identified tonoplast proteins in our present study provide an informative basis for arsenic carriers or channels and help to clarify the regulation of tonoplast arsenic transport processes in P. vittata. Biological significance Vacuole compartmentalization is crucial to As hyperaccumulator P. vittata, while there is limited known arsenic transport proteins involved in vacuole compartmentalization. In this paper, we obtained tonoplast of P. vittata fronds by iodixanol step gradient centrifugation method and then used TMPP protein labeling proteome technology for the quantitative detection of fronds tonoplast proteins. Our findings are the first challenge to the tonoplast proteins data mining of P. vittata which provide an informative basis for As carriers or channels. The proteomic approach in our study is suited for detecting alterations tonoplast protein and help to clarify the regulation of tonoplast transport processes. This article is part of a Special Issue entitled: Proteomics of non-model organisms. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods |
| 电子版国际标准刊号 | 1876-7737 |
| 出版地 | AMSTERDAM |
| WOS关键词 | EMBRYONIC STEM-CELLS ; SACCHAROMYCES-CEREVISIAE ; OXIDATIVE STRESS ; CHINESE BRAKE ; ARABIDOPSIS ; TRANSPORTER ; TOLERANCE ; MECHANISMS ; SPECIATION ; ATPASE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000338600000005 |
| 资助机构 | National Natural Science Foundation of China [31070449, 31370299] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27226]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Hongling,He, Zhenyan,Yan, Huili,et al. The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.[J]. JOURNAL OF PROTEOMICS,2014,105:46-57. |
| APA | Shen, Hongling.,He, Zhenyan.,Yan, Huili.,Xing, Zenan.,Chen, Yanshan.,...&Ma, Mi.(2014).The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L..JOURNAL OF PROTEOMICS,105,46-57. |
| MLA | Shen, Hongling,et al."The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.".JOURNAL OF PROTEOMICS 105(2014):46-57. |
入库方式： OAI收割
来源：植物研究所
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