RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence
文献类型：期刊论文
| 作者 | Lu, Peitao; Zhang, Changqing; Liu, Jitao; Liu, Xiaowei; Jiang, Guimei; Jiang, Xinqiang; Khan, Muhammad Ali; Wang, Liangsheng1 ; Hong, Bo; Gao, Junping
|
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2014 |
| 卷号 | 78期号:4页码:578-590 |
| 关键词 | Rosa hybrida flower senescence RhHB1 gibberellins abscisic acid ethylene |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.12494 |
| 文献子类 | Article |
| 英文摘要 | Rose (Rosa hybrida) is one of the most important ornamental plants worldwide; however, senescence of its petals terminates the ornamental value of the flower, resulting in major economic loss. It is known that the hormones abscisic acid (ABA) and ethylene promote petal senescence, while gibberellins (GAs) delay the process. However, the molecular mechanisms underlying the antagonistic effects amongst plant hormones during petal senescence are still unclear. Here we isolated RhHB1, a homeodomain-leucine zipper I transcription factor gene, from rose flowers. Quantitative RT-PCR and GUS reporter analyses showed that RhHB1 was strongly expressed in senescing petals, and its expression was induced by ABA or ethylene in petals. ABA or ethylene treatment clearly accelerated rose petal senescence, while application of the gibberellin GA(3) delayed the process. However, silencing of RhHB1 delayed the ABA- or ethylene-mediated senescence, and resulted in higher petal anthocyanin levels and lower expression of RhSAG12. Moreover, treatment with paclobutrazol, an inhibitor of GA biosynthesis, repressed these delays. In addition, silencing of RhHB1 blocked the ABA- or ethylene-induced reduction in expression of the GA20 oxidase encoded by RhGA20ox1, a gene in the GA biosynthetic pathway. Furthermore, RhHB1 directly binds to the RhGA20ox1 promoter, and silencing of RhGA20ox1 promoted petal senescence. Eight senescence-related genes showed substantial differences in expression in petals after treatment with GA(3) or paclobutrazol. These results suggest that RhHB1 mediates the antagonistic effect of GAs on ABA and ethylene during rose petal senescence, and that the promotion of petal senescence by ABA or ethylene operates through an RhHB1-RhGA20ox1 regulatory checkpoint. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | HD-ZIP PROTEIN ; ABSCISIC-ACID ; LEAF SENESCENCE ; GENE-EXPRESSION ; PERIANTH SENESCENCE ; SIGNALING PATHWAYS ; PETUNIA FLOWERS ; ARABIDOPSIS ; CARNATION ; HOMEODOMAIN |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000335537600004 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China [31130048, 31171992, 31071827] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27252]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Peitao,Zhang, Changqing,Liu, Jitao,et al. RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence[J]. PLANT JOURNAL,2014,78(4):578-590. |
| APA | Lu, Peitao.,Zhang, Changqing.,Liu, Jitao.,Liu, Xiaowei.,Jiang, Guimei.,...&Gao, Junping.(2014).RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence.PLANT JOURNAL,78(4),578-590. |
| MLA | Lu, Peitao,et al."RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence".PLANT JOURNAL 78.4(2014):578-590. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。