The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility
文献类型：期刊论文
| 作者 | Zhou, Qingyuan; Jia, Junting; Huang, Xing; Yan, Xueqing1; Cheng, Liqin; Chen, Shuangyan; Li, Xiaoxia; Peng, Xianjun; Liu, Gongshe
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| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2014 |
| 卷号 | 15 |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/1471-2164-15-399 |
| 文献子类 | Article |
| 英文摘要 | Background: Many Poaceae species show a gametophytic self-incompatibility (GSI) system, which is controlled by at least two independent and multiallelic loci, S and Z. Until currently, the gene products for S and Z were unknown. Grass SI plant stigmas discriminate between pollen grains that land on its surface and support compatible pollen tube growth and penetration into the stigma, whereas recognizing incompatible pollen and thus inhibiting pollination behaviors. Leymus chinensis (Trin.) Tzvel. (sheepgrass) is a Poaceae SI species. A comprehensive analysis of sheepgrass stigma transcriptome may provide valuable information for understanding the mechanism of pollen-stigma interactions and grass SI. Results: The transcript abundance profiles of mature stigmas, mature ovaries and leaves were examined using high-throughput next generation sequencing technology. A comparative transcriptomic analysis of these tissues identified 1,025 specifically or preferentially expressed genes in sheepgrass stigmas. These genes contained a significant proportion of genes predicted to function in cell-cell communication and signal transduction. We identified 111 putative transcription factors (TFs) genes and the most abundant groups were MYB, C2H2, C3H, FAR1, MADS. Comparative analysis of the sheepgrass, rice and Arabidopsis stigma-specific or preferential datasets showed broad similarities and some differences in the proportion of genes in the Gene Ontology (GO) functional categories. Potential SI candidate genes identified in other grasses were also detected in the sheepgrass stigma-specific or preferential dataset. Quantitative real-time PCR experiments validated the expression pattern of stigma preferential genes including homologous grass SI candidate genes. Conclusions: This study represents the first large-scale investigation of gene expression in the stigmas of an SI grass species. We uncovered many notable genes that are potentially involved in pollen-stigma interactions and SI mechanisms, including genes encoding receptor-like protein kinases (RLK), CBL (calcineurin B-like proteins) interacting protein kinases, calcium-dependent protein kinase, expansins, pectinesterase, peroxidases and various transcription factors. The availability of a pool of stigma-specific or preferential genes for L. chinensis offers an opportunity to elucidate the mechanisms of SI in Poaceae. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 出版地 | LONDON |
| WOS关键词 | POLLEN-TUBE GROWTH ; SQUALIDUS L. ASTERACEAE ; CELL-WALL EXTENSION ; TRANSCRIPTION FACTORS ; ARABIDOPSIS-THALIANA ; GENOME ANNOTATION ; BAX INHIBITOR-1 ; PAPAVER-RHOEAS ; PROTEIN-KINASE ; ABSCISIC-ACID |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000336682800001 |
| 出版者 | BMC |
| 资助机构 | National Basic Research Program of China (973) [2014CB138704] ; National Natural Science Foundation of China [31370611] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27366]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Beijing Comp Ctr, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qingyuan,Jia, Junting,Huang, Xing,et al. The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility[J]. BMC GENOMICS,2014,15. |
| APA | Zhou, Qingyuan.,Jia, Junting.,Huang, Xing.,Yan, Xueqing.,Cheng, Liqin.,...&Liu, Gongshe.(2014).The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility.BMC GENOMICS,15. |
| MLA | Zhou, Qingyuan,et al."The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility".BMC GENOMICS 15(2014). |
入库方式： OAI收割
来源：植物研究所
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