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Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding

文献类型：期刊论文

作者Chen, Shuangyan; Cai, Yueyue1; Zhang, Lexin2; Yan, Xueqing1; Cheng, Liqin; Qi, Dongmei; Zhou, Qingyuan; Li, Xiaoxia; Liu, Gongshe
刊名PLOS ONE
出版日期2014
卷号9期号:2
ISSN号1932-6203
DOI10.1371/journal.pone.0089495
文献子类Article
英文摘要Background: Herbivore grazing is a multiple-component process that includes wounding, defoliation, and saliva deposition. Despite the extensive published research on mechanical wounding and defoliation, no analysis to identify the genes that specify defoliation and mechanical wounding has been performed. Moreover, the influence of the expression of these genes on plant regrowth after defoliation remains poorly understood. Results: Seven cDNA libraries for RNA samples collected from stubble tissues that had been mechanically wounded or defoliated at 2, 6 and 24 h along with the control were sequenced using the Illumina/Solexa platform. A comparative transcriptomic analysis of the sequencing data was conducted. In total, 1,836 and 3,238 genes were detected with significant differential expression levels after wounding and defoliation, respectively, during one day. GO, KOG and pathway-based enrichment analyses were performed to determine and further understand the biological functions of those differentially expressed genes (DEGs). The results demonstrated that both wounding and defoliation activated the systemic synthesis of jasmonate (JA). However, defoliation specifically reduced the expression levels of ribosomal protein genes, cell division or cell expansion-related genes, and lignin biosynthesis genes and may have negatively affected plant growth. Further analysis revealed that the regrowth of elongating leaves was significantly retarded after defoliation at 6 h through the following 7 days of measurement, suggesting that the gene expression pattern and phenotype are consistent. Fifteen genes were selected, and their expression levels were confirmed by quantitative RT-PCR (qRT-PCR). Thirteen of them exhibited expression patterns consistent with the digital gene expression (DGE) data. Conclusions: These sequencing datasets allowed us to elucidate the common and distinct mechanisms of plant responses to defoliation and wounding. Additionally, the distinct DEGs represent a valuable resource for novel gene discovery that may improve plant resistance to defoliation from various processes.
学科主题Multidisciplinary Sciences
出版地SAN FRANCISCO
WOS关键词DOWN-REGULATION ; PERENNIAL RYEGRASS ; LOLIUM-PERENNE ; RNA-SEQ ; BIOSYNTHESIS ; EXPRESSION ; ARABIDOPSIS ; HERBIVORY ; LIGNIN ; GROWTH
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000331717900106
出版者PUBLIC LIBRARY SCIENCE
资助机构National Basic Research Program of China (973'') [2014CB138704] ; National High Technology Research and Development Program of China (863'') [2011AA100209] ; Ningxia Agricultural Comprehensive Development Office [NTKJ-2013-03 (1), NTKJ-2013-04] ; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-B-05]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27369]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
2.Chinese Acad Sci, Grad Schoo1, Beijing, Peoples R China
3.Entry Exit Inspect & Quarantine Bur, Heze, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Shuangyan,Cai, Yueyue,Zhang, Lexin,et al. Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding[J]. PLOS ONE,2014,9(2).
APA Chen, Shuangyan.,Cai, Yueyue.,Zhang, Lexin.,Yan, Xueqing.,Cheng, Liqin.,...&Liu, Gongshe.(2014).Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding.PLOS ONE,9(2).
MLA Chen, Shuangyan,et al."Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding".PLOS ONE 9.2(2014).

入库方式： OAI收割

来源：植物研究所

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