Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes
文献类型：期刊论文
| 作者 | Xie Lei; Wang Ying Wei2; Guan Shan Yue3; Xie Li Jing1; Long Xin1; Sun Cheng Ye1 |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2014 |
| 卷号 | 27期号:10页码:794-806 |
| 关键词 | Poisonous plants DNA barcoding matK rbcL ITS |
| ISSN号 | 0895-3988 |
| DOI | 10.3967/bes2014.115 |
| 文献子类 | Article |
| 英文摘要 | Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes (matK, rbcL, and ITS) were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment (PWG) distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocallis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method. Conclusion DNA barcoding technique provides us a fast tool for clinical identification of poisonous plants in China. We suggest matK, rbcL, ITS used in combination as DNA barcodes for authentication of poisonous plants. |
| 学科主题 | Environmental Sciences ; Public, Environmental & Occupational Health |
| 出版地 | BEIJING |
| 电子版国际标准刊号 | 2214-0190 |
| WOS关键词 | SPECIES IDENTIFICATION ; LAND PLANTS ; RANUNCULACEAE ; IDENTIFY ; ACONITUM ; DELPHINIUM ; PHYLOGENY ; UTILITY |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:000344175900006 |
| 出版者 | CHINESE CENTER DISEASE CONTROL & PREVENTION |
| 资助机构 | Fundamental Research Funds for the Central Universities [TD2012-04] ; Scientific Research Foundation of the State Human Resource Ministry ; Education Ministry for Returned Chinese Scholars ; State Key Laboratory of Systematic and Evolutionary Botany [LSEB2011-07] ; Main Direction Program of Knowledge Innovation of the Chinese Academy of Sciences [KSCX2-EW-Z-1] ; National Natural Science Foundation of China [31170201, 81072317, 31110103911] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27285]  |
| 专题 | 植物园 |
| 作者单位 | 1.Beijing Forestry Univ, Coll Nat Conservat, Beijing 100083, Peoples R China 2.Natl Inst Occupat Hlth & Poison Control, China Ctr Dis Control & Prevent, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Clemson Univ, Sch Agr Forestry & Environm Sci Forest Resources, Clemson, SC 29634 USA |
| 推荐引用方式 GB/T 7714 | Xie Lei,Wang Ying Wei,Guan Shan Yue,et al. Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2014,27(10):794-806. |
| APA | Xie Lei,Wang Ying Wei,Guan Shan Yue,Xie Li Jing,Long Xin,&Sun Cheng Ye.(2014).Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,27(10),794-806. |
| MLA | Xie Lei,et al."Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 27.10(2014):794-806. |
入库方式： OAI收割
来源：植物研究所
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