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  2. 地质与地球物理研究所
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  4. 中国科学院地球与行星物理重点实验室
Distinct Microbial Communities in the Soils, Waters, and Speleothems of a Hyperalkaline Cave System

文献类型：期刊论文

作者Shen, Jianxun2,3,5; Smith, Andrew C.4; Barnett, Megan J.4; Morgan, Alistair1; Wynn, Peter M.1
刊名JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES
出版日期2022-09-01
卷号127期号:9页码:17
ISSN号2169-8953
关键词Poole's Cavern karst hyperalkaline taxonomy metabolism inference
DOI10.1029/2022JG006866
英文摘要Caves are complex ecosystems with various microbial habitats. Understanding the individual community structures in the key source environments (soils, waters) and those in the sinks (speleothems, cave muds) can help elucidate the extent to which in-cave communities are a function of their source communities, or if they can successfully adapt and diversify locally. Here, we assess the network of microbial communities existing within a unique British cave system. Poole's Cavern is characterized by alkalinity or even hyperalkalinity (pH > 9) in its drip waters, creating a series of challenging ecological niches for microbes to survive. Samples of soil, drip water, speleothem, and cave sediment were collected for chemical characterization, cell culture assays and DNA sequencing analysis. We show that microbial abundance and diversity were highest in the soils proximal to the cave, and the microbiotas of the hyperalkaline cave chamber were less abundant and less diverse than the cave chamber with lower pH. Proteobacteria and Planctomycetota were the most prevalent bacterial phyla throughout the cave system irrespective of pH, indicating their members are comparatively more metabolically versatile. Predicted essential metabolic pathways were still dominant within all sampling sites, and microorganisms were shown to be capable of utilizing various inorganic or simple organic compounds to survive. Interestingly, co-occurrence between Poole's samples was limited, never demonstrating more than 50% similarity. This work highlights the diversity of microbial communities within this extreme environment and the development of microbial niches which reflects the adaptation strategy of microorganisms under alkaline-hyperalkaline conditions. Plain Language Summary Caves are complex environments, forming a range of living spaces for microbes. Dissolved inorganic minerals and organic matter are carried by water that flows from the soils above caves to speleothems and into sediments on the ground. Poole's Cavern in the UK is a cave system with very high pH (>9) waters. These waters are not friendly to most microbes living in neutral pH environments and thus possibly foster special microbial communities that can stay alive under high pH conditions. Full of calcite formations and without observable fauna, this cave is not rich in nutrients. We sampled soils above Poole's Cavern, drip waters, speleothems, and cave muds to determine the biomass and microbial species in them. We found that the soils outside of this cave had the highest microbial content and diversity. The cave chamber with higher pH waters had fewer microbes than that with lower pH waters. We demonstrate that connectivity of microbial species between in-cave sampling sites was limited, and the community of microbes in each site developed relatively independent finding ways to survive in their respective environment. This study presents the high potential of microbes to evolve and adapt even in conditions almost inhospitable to life.
WOS关键词KARTCHNER CAVERNS ; POOLES CAVERN ; GEN. NOV. ; DIVERSITY ; BACTERIAL ; CARBON ; SILVA
资助项目BCRA Cave Science and Technology Research Fund ; British Geological Survey ; Lancaster University ; Buxton Civic Association
WOS研究方向Environmental Sciences & Ecology ; Geology
语种英语
出版者AMER GEOPHYSICAL UNION
WOS记录号WOS:000853448100001
资助机构BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; British Geological Survey ; British Geological Survey ; British Geological Survey ; British Geological Survey ; Lancaster University ; Lancaster University ; Lancaster University ; Lancaster University ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; British Geological Survey ; British Geological Survey ; British Geological Survey ; British Geological Survey ; Lancaster University ; Lancaster University ; Lancaster University ; Lancaster University ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; British Geological Survey ; British Geological Survey ; British Geological Survey ; British Geological Survey ; Lancaster University ; Lancaster University ; Lancaster University ; Lancaster University ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; BCRA Cave Science and Technology Research Fund ; British Geological Survey ; British Geological Survey ; British Geological Survey ; British Geological Survey ; Lancaster University ; Lancaster University ; Lancaster University ; Lancaster University ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association ; Buxton Civic Association
源URL[http://ir.iggcas.ac.cn/handle/132A11/108373]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Shen, Jianxun
作者单位1.Univ Lancaster, Lancaster Environm Ctr, Lancaster, England
2.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
3.Univ St Andrews, Sch Earth & Environm Sci, St Andrews, Fife, Scotland
4.British Geol Survey, Environm Sci Ctr, Nottingham, England
5.Univ St Andrews, Ctr Exoplanet Sci, St Andrews, Fife, Scotland
推荐引用方式
GB/T 7714		 Shen, Jianxun,Smith, Andrew C.,Barnett, Megan J.,et al. Distinct Microbial Communities in the Soils, Waters, and Speleothems of a Hyperalkaline Cave System[J]. JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES,2022,127(9):17.
APA Shen, Jianxun,Smith, Andrew C.,Barnett, Megan J.,Morgan, Alistair,&Wynn, Peter M..(2022).Distinct Microbial Communities in the Soils, Waters, and Speleothems of a Hyperalkaline Cave System.JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES,127(9),17.
MLA Shen, Jianxun,et al."Distinct Microbial Communities in the Soils, Waters, and Speleothems of a Hyperalkaline Cave System".JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES 127.9(2022):17.

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来源：地质与地球物理研究所

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