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  4. 中国科学院地球与行星物理重点实验室
An electrochemical system for the rapid and accurate quantitation of microbial exoelectrogenic ability

文献类型：期刊论文

作者Wang, Huan; Zheng, Yue2,6,7; Liu, Jiawei5; Zhu, Baoli4; Qin, Wei3; Zhao, Feng1,6
刊名BIOSENSORS & BIOELECTRONICS
出版日期2022-11-01
卷号215页码:7
ISSN号0956-5663
关键词Microbial electrochemical technology Electroactive microorganisms Extracellular electron transfer Au electrode Shewanella
DOI10.1016/j.bios.2022.114584
英文摘要Microbial extracellular electron transfer (EET) plays a vital role in globally important environmental phenomena, including bioremediation, bioenergy generation, and biofuel production. The quantitation of microbial exoe-lectrogenic ability is fundamental to studying the process of EET. However, there is no accurate and time-saving protocol to directly evaluate EET ability, hindering our understanding and application of EET. In this work, we proposed an accurate and rapid quantitation system for measuring EET ability using a gold-coated membrane filter as a working electrode. The quantitation signals could be recorded within 1 h and accurately normalized by the number of cells with outstanding repeatability and reproducibility. Further, this method could be distin-guished microbial direct EET performances of different growth stages, and the results showed the middle log-arithmic growth stage of Shewanella onedensis MR-1 had the best electrochemical activity. This method can be widely used for different types of electroactive microorganisms, including gram-negative bacteria, gram-positive bacteria, and fungi. Due to its time savings, accurate quantification and easy operation, this method provides a standard way to assess the role of EET ability.
WOS关键词EXTRACELLULAR ELECTRON-TRANSFER ; BACTERIA ; GROWTH
资助项目National Natural Science Foundation of China[41907027] ; National Natural Science Foundation of China[22025603] ; Fujian Provincial Department of Science and Technology[2021J01529]
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
出版者ELSEVIER ADVANCED TECHNOLOGY
WOS记录号WOS:000847850100001
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fujian Provincial Department of Science and Technology ; Fujian Provincial Department of Science and Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fujian Provincial Department of Science and Technology ; Fujian Provincial Department of Science and Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fujian Provincial Department of Science and Technology ; Fujian Provincial Department of Science and Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fujian Provincial Department of Science and Technology ; Fujian Provincial Department of Science and Technology
源URL[http://ir.iggcas.ac.cn/handle/132A11/108395]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Zheng, Yue; Zhao, Feng
作者单位1.Chinese Acad Sci, Inst Urban Environm, 1799 Jimei Rd, Xiamen, Peoples R China
2.Xiamen Univ, Coll Environm & Ecol, State Key Lab Marine Environm Sci, Xiamen, Peoples R China
3.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK USA
4.Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Taoyuan Agroecosystem Res Stn, Changsha 410125, Peoples R China
5.Chinese Acad Sci, Inst Geol & Geophys, Innovat Acad Earth Sci, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China
6.Chinese Acad Sci, Inst Urban Environm, CAS Key Lab Urban Pollutant Convers, Xiamen 361021, Peoples R China
7.Xiamen Univ, Coll Environm & Ecol, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Huan,Zheng, Yue,Liu, Jiawei,et al. An electrochemical system for the rapid and accurate quantitation of microbial exoelectrogenic ability[J]. BIOSENSORS & BIOELECTRONICS,2022,215:7.
APA Wang, Huan,Zheng, Yue,Liu, Jiawei,Zhu, Baoli,Qin, Wei,&Zhao, Feng.(2022).An electrochemical system for the rapid and accurate quantitation of microbial exoelectrogenic ability.BIOSENSORS & BIOELECTRONICS,215,7.
MLA Wang, Huan,et al."An electrochemical system for the rapid and accurate quantitation of microbial exoelectrogenic ability".BIOSENSORS & BIOELECTRONICS 215(2022):7.

入库方式： OAI收割

来源：地质与地球物理研究所

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