中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Hinge region mediates signal transmission of luteinizing hormone and chorionic gonadotropin receptor

文献类型：期刊论文

作者He, Xinheng3,4,5; Duan, Jia3,4,5; Ji, Yujie3,4,5; Zhao, Lifen3,4; Jiang, Hualiang1,2,3,4,5,6; Jiang, Yi2,3,4,6; Xu, H. Eric3,4,5; Cheng, Xi1,3,4,5
刊名COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
出版日期2022
卷号20页码:6503-6511
关键词Glycoprotein hormone receptors G protein -coupled receptors Molecular dynamics simulation Protein dynamics Evolutionary analysis
ISSN号2001-0370
DOI10.1016/j.csbj.2022.11.039
通讯作者Jiang, Yi(yjiang@lglab.ac.cn) ; Xu, H. Eric(eric.xu@simm.ac.cn) ; Cheng, Xi(xicheng@simm.ac.cn)
英文摘要Luteinizing hormone-choriogonadotropin receptor (LHCGR), a class A G protein-coupled receptor (GPCR), plays a pivotal role in the maturation of reproductive organs and embryonic development. Compared with other GPCRs, the subfamily of LHCGR has a large extracellular domain (ECD) to interact with glyco-protein hormones. A unique hinge region connects the ECD and transmembrane domain (TMD) to trans-fer the activation signal. However, the signal transmission mechanism remains largely unknown. Here, both molecular dynamics simulation and evolutional analysis were applied to explore the effect of the hinge region on signal transmission. The glycoprotein hormone determined specific hinge region confor-mations, including the position of a long hinge loop and the ECD-TMD interface. With the hormone, the hinge region showed a characteristic rotation and displayed an active-like conformational landscape of the ECD-TMD interface with an extended TMD. The active-like hinge region conformation transduces the hormone binding signal downwards from ECD to TMD. The relationship between the hinge region and the intracelluar G protein-binding pocket was also inferred. The hinge region-mediated signal trans-mission mechanism offers a deeper understanding of LHCGR and provides insights into the elucidation of GPCR activation.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).
WOS关键词PROTEIN ; DYNAMICS ; RECOGNITION ; ACTIVATION ; MECHANISM ; SIMULATIONS ; ECTODOMAIN ; DIVERSITY ; MUTATION ; DOMAIN
资助项目Shanghai Municipal Science and Technology Major Project ; Lin-gang Laboratory grant[LG202102-01-01] ; Ministry of Science and Technology (China)[2018YFA0507002] ; CAS Strategic Priority Research Program[XDB37030103] ; National Natural Science Foundation of China[32130022] ; National Natural Science Foundation of China[32171187] ; National Natural Science Foundation of China[82121005]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000913258100010
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/303382]  
专题新药研究国家重点实验室
通讯作者Jiang, Yi; Xu, H. Eric; Cheng, Xi
作者单位1.Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou, Peoples R China
2.Lingang Lab, Shanghai, Peoples R China
3.Chinese Acad Sci, State Key Lab Drug Res, Shanghai, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 He, Xinheng,Duan, Jia,Ji, Yujie,et al. Hinge region mediates signal transmission of luteinizing hormone and chorionic gonadotropin receptor[J]. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,2022,20:6503-6511.
APA He, Xinheng.,Duan, Jia.,Ji, Yujie.,Zhao, Lifen.,Jiang, Hualiang.,...&Cheng, Xi.(2022).Hinge region mediates signal transmission of luteinizing hormone and chorionic gonadotropin receptor.COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,20,6503-6511.
MLA He, Xinheng,et al."Hinge region mediates signal transmission of luteinizing hormone and chorionic gonadotropin receptor".COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 20(2022):6503-6511.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。