Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T 1-MR imaging, and antitumor immunity
文献类型：期刊论文
| 作者 | Hou, Yike2; Tang, Zhe2; Farheen, Jabeen2; Saeed, Madiha3,4; Luo, Lijia5; Ren, Wenzi5; Luo, Dandan2; Mushtaq, Asim2; Zhao, Ruibo2,6; Ge, Jian1 |
| 刊名 | JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2023-06-10 |
| 卷号 | 148页码:123-137 |
| ISSN号 | 1005-0302 |
| 关键词 | Ovalbumin Nanovaccine Antigen delivery Magnetic resonance imaging Contrast agent Cancer immunotherapy |
| DOI | 10.1016/j.jmst.2022.11.024 |
| 通讯作者 | Zhao, Ruibo(rzhao@zstu.edu.cn) ; Iqbal, M. Zubair(zubair@zstu.edu.cn) ; Kong, Xiangdong(kongxd@zstu.edu.cn) |
| 英文摘要 | Vaccine-based cancer immunotherapy has demonstrated a significant potential for cancer treatment in clinics. Although the efficiencies of vaccines are limited, they can be enhanced by a well-designed antigen delivery system that promotes sufficient antigen presentation of dendritic cells (DCs) for initiating high T cell immunity. Herein, antigen-loaded manganese oxide (Mn3O4) triangular-shaped ultrasmall nanoparti-cles were prepared to stimulate DC-based immunotherapy under the guidance of T 1 magnetic resonance imaging. The FDA-approved triblock copolymer Pluronic (R) F-68 was used not only to transfer the phase from hydrophobic to hydrophilic but also to enrich antigen loading and improve the biocompatibility of the prepared nanoparticles. Ovalbumin (OVA), a model antigen, was adsorbed on the surface of polymer-coated nanoparticles through electrostatic interaction to form Mn3O4 @PF68-OVA nanoparticle-antigen complexes to stimulate DC-based immunization and antigen-specific T cell immunity. The Mn3O4 @PF68-OVA nanovaccine (NV) induces negligible toxicity effects against 4T1 and bone marrow-derived dendritic cells (BMDCs) by conventional methods supports the proliferation of intestine organoids, which are an innovative three-dimensional cytotoxicity evaluation system, thereby indicating their potential safety for in vivo cancer therapies. The designed paramagnetic nanovaccine possessed excellent OVA delivery to dendritic-regulated antigen-specific T cells in vitro by stimulating the maturation level of BMDCs. In ad-dition, Mn3O4 @PF68-OVA NVs enhance immunity in vivo by increasing the T-cells and M1 macrophages, which suggests improved immunity. Excitingly, vaccination with Mn3O4 @PF68-OVA offer complete pro-tection in the prophylactic group and significant tumor inhibition in the therapeutic group against B16 -OVA tumor. In addition, the designed nanovaccine demonstrated high T 1-MR imaging in the tumor, fur-ther justifying enhanced tumor accumulation and capability to real-time monitor the treatment proce-dure. This study presents a promising nanosystem to design an effective nanovaccine for T 1-MR imaging-guided tumor immunotherapy.(c) 2023 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology. |
| WOS关键词 | CONTRAST AGENT ; CANCER-IMMUNOTHERAPY ; CULTURE METHOD ; NANOPARTICLES |
| 资助项目 | National Natural Science Foundation of China[51672250] ; National Natural Science Foundation of China[51902289] ; Zhejiang International Science and Technology Cooperation Project[2021C01180] ; Zhejiang International Science and Technology Cooperation Project[2019C04020] ; Research Foundation of ZSTU[18012134-Y] |
| WOS研究方向 | Materials Science ; Metallurgy & Metallurgical Engineering |
| 语种 | 英语 |
| 出版者 | JOURNAL MATER SCI TECHNOL |
| WOS记录号 | WOS:000920656400001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/303693]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zhao, Ruibo; Iqbal, M. Zubair; Kong, Xiangdong |
| 作者单位 | 1.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China 2.Zhejiang Sci Tech Univ, Inst Smart Biomed Mat, Sch Mat Sci & Engn, Hangzhou 310018, Peoples R China 3.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Cixi Inst Biomed Engn, Ningbo Inst Mat Technol & Engn, CAS Key Lab Magnet Mat & Devices,Key Lab Addit Mfg, Ningbo 315201, Peoples R China 6.Imperial Coll London, Dept Mat, London SW7 2AZ, England 7.Shaoxing Univ, Med Sci Res Ctr, Sch Med, Lab Nanomed, Shaoxing 312000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Yike,Tang, Zhe,Farheen, Jabeen,et al. Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T 1-MR imaging, and antitumor immunity[J]. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,2023,148:123-137. |
| APA | Hou, Yike.,Tang, Zhe.,Farheen, Jabeen.,Saeed, Madiha.,Luo, Lijia.,...&Kong, Xiangdong.(2023).Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T 1-MR imaging, and antitumor immunity.JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,148,123-137. |
| MLA | Hou, Yike,et al."Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T 1-MR imaging, and antitumor immunity".JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY 148(2023):123-137. |
入库方式： OAI收割
来源：上海药物研究所
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