Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2
文献类型：期刊论文
| 作者 | Xia, Bingqing2,3; Pan, Xiaoyan1; Luo, Rong-Hua4; Shen, Xurui2,3; Li, Shuangqu2,3; Wang, Yi2,3; Zuo, Xiaoli2; Wu, Yan1; Guo, Yingqi5; Xiao, Gengfu1 |
| 刊名 | CELL DISCOVERY
 |
| 出版日期 | 2023-01-06 |
| 卷号 | 9期号:1页码:15 |
| DOI | 10.1038/s41421-022-00510-2 |
| 通讯作者 | Xia, Bingqing(xiabingqing@simm.ac.cn) ; Zheng, Yong-Tang(zhengyt@mail.kiz.ac.cn) ; Li, Jia(jli@simm.ac.cn) ; Zhang, Lei-Ke(zhangleike@wh.iov.cn) ; Gao, Zhaobing(zbgao@simm.ac.cn) |
| 英文摘要 | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic. Antibody resistance dampens neutralizing antibody therapy and threatens current global Coronavirus (COVID-19) vaccine campaigns. In addition to the emergence of resistant SARS-CoV-2 variants, little is known about how SARS-CoV-2 evades antibodies. Here, we report a novel mechanism of extracellular vesicle (EV)-mediated cell-to-cell transmission of SARS-CoV-2, which facilitates SARS-CoV-2 to escape from neutralizing antibodies. These EVs, initially observed in SARS-CoV-2 envelope protein-expressing cells, are secreted by various SARS-CoV-2-infected cells, including Vero E6, Calu-3, and HPAEpiC cells, undergoing infection-induced pyroptosis. Various SARS-CoV-2-infected cells produce similar EVs characterized by extra-large sizes (1.6-9.5 mu m in diameter, average diameter > 4.2 mu m) much larger than previously reported virus-generated vesicles. Transmission electron microscopy analysis and plaque assay reveal that these SARS-CoV-2-induced EVs contain large amounts of live virus particles. In particular, the vesicle-cloaked SARS-CoV-2 virus is resistant to neutralizing antibodies and able to reinfect naive cells independent of the reported receptors and cofactors. Consistently, the constructed 3D images show that intact EVs could be taken up by recipient cells directly, supporting vesicle-mediated cell-to-cell transmission of SARS-CoV-2. Our findings reveal a novel mechanism of receptor-independent SARS-CoV-2 infection via cell-to-cell transmission, provide new insights into antibody resistance of SARS-CoV-2 and suggest potential targets for future antiviral therapeutics. |
| WOS关键词 | EXOSOMES ; MITOCHONDRIA ; BIOGENESIS ; PYROPTOSIS ; MECHANISMS ; SECRETION |
| 资助项目 | National Science Fund of Distinguished Young Scholars[81825021] ; Fund of Youth Innovation Promotion Association[2019285] ; Fund of Youth Innovation Promotion Association[2021333] ; National Natural Science Foundation of China[81773707] ; National Natural Science Foundation of China[31700732] ; Shanghai Municipal Science and Technology Major Project, ; Fund of Shanghai Science and Technology Innovation Action Plan[20ZR1474200] ; Shanghai Rising-Star Program[22QA1411000] ; National Key Laboratory Program of China[LG202101-01-04] ; National Key Research and Development Program of China[2020YFC0842000] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences[XDA12050308] ; Fund of National Science and Technology Major Project[2018ZX09711002-002-006] ; Hubei Science and Technology Project[2020FCA003] ; Yunnan Key Research and Development Program[202103AC100001] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| 出版者 | SPRINGERNATURE |
| WOS记录号 | WOS:000910150600001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/303799]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xia, Bingqing; Zheng, Yong-Tang; Li, Jia; Zhang, Lei-Ke; Gao, Zhaobing |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Stake Key Lab Drug Res, Shanghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Kunming Inst Zool, Chinese Acad Sci & Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Zool, Publ Technol Serv Ctr, Kunming, Yunnan, Peoples R China 6.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming, Yunnan, Peoples R China 7.Chinese Acad Sci, Inst Drug Discovery Innovat, Zhongshan Inst Drug Res, Zhongshan, Guangdong, Peoples R China 8.Fudan Univ, Sch Pharm, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Bingqing,Pan, Xiaoyan,Luo, Rong-Hua,et al. Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2[J]. CELL DISCOVERY,2023,9(1):15. |
| APA | Xia, Bingqing.,Pan, Xiaoyan.,Luo, Rong-Hua.,Shen, Xurui.,Li, Shuangqu.,...&Gao, Zhaobing.(2023).Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2.CELL DISCOVERY,9(1),15. |
| MLA | Xia, Bingqing,et al."Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2".CELL DISCOVERY 9.1(2023):15. |
入库方式： OAI收割
来源：上海药物研究所
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