Multiplication of defective Ebola virus in a complementary permissive cell line
文献类型：期刊论文
| 作者 | Tong, Xian-Kun1 ; Li, Heng1,4; Yang, Li1; Xie, Shi-Zhe2,4; Xie, Sha1; Gong, Ying1,4; Peng, Cheng3; Gao, Xiao-Xiao3; Shi, Zheng-Li2; Yang, Xing-Lou2,5,6
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| 刊名 | ANTIVIRAL RESEARCH
 |
| 出版日期 | 2023 |
| 卷号 | 209页码:8 |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2022.105491 |
| 通讯作者 | Tong, Xian-Kun(xktong@simm.ac.cn) ; Yang, Xing-Lou(yangxinglou@mail.kiz.ac.cn) ; Zuo, Jian-Ping(jpzuo@simm.ac.cn) |
| 英文摘要 | In an effort to develop safe and innovative in vitro models for Ebola virus (EBOV) research, we generated a recombinant Ebola virus where the glycoprotein (GP) gene was substituted with the Cre recombinase (Cre) gene by reverse genetics. This defective virus could multiply itself in a complementary permissive cell line, which could express GP and reporter protein upon exogenous Cre existence. The main features of this novel model for Ebola virus are intact viral life cycle, robust virus multiplication and normal virions morphology. The design of this model ensures its safety, excellent stability and maneuverability as a tool for virology research as well as for antiviral agent screening and drug discovery, and such a design could be further adapted to other viruses. |
| WOS关键词 | DNA ; SYSTEM ; GENOME ; RECOMBINATION ; GLYCOPROTEIN ; REPLICATION ; RELEVANCE ; MARBURG ; ENTRY |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000908932700001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/303893]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Tong, Xian-Kun; Yang, Xing-Lou; Zuo, Jian-Ping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Immunol Dis Res Ctr, State Key Lab Drug Res,BSL 3 Lab, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, Wuhan 430071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Hubei Jiangxia Lab, Wuhan 430071, Peoples R China 6.Chinese Acad Sci, Kunming Inst Zool, Yunnan Key Lab Biodivers Informat, Kunming 650023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tong, Xian-Kun,Li, Heng,Yang, Li,et al. Multiplication of defective Ebola virus in a complementary permissive cell line[J]. ANTIVIRAL RESEARCH,2023,209:8. |
| APA | Tong, Xian-Kun.,Li, Heng.,Yang, Li.,Xie, Shi-Zhe.,Xie, Sha.,...&Zuo, Jian-Ping.(2023).Multiplication of defective Ebola virus in a complementary permissive cell line.ANTIVIRAL RESEARCH,209,8. |
| MLA | Tong, Xian-Kun,et al."Multiplication of defective Ebola virus in a complementary permissive cell line".ANTIVIRAL RESEARCH 209(2023):8. |
入库方式： OAI收割
来源：上海药物研究所
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