Development and validation of a LC-MS/MS method for quantitation of recombinant human growth hormone in rat plasma and application to a pharmacokinetic study
文献类型：期刊论文
| 作者 | Huang, Wen-si1,2; Yu, Jing-hua2; Diao, Xing-xing1,2 |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2023-02-05 |
| 卷号 | 224页码:9 |
| 关键词 | Recombinant human growth hormone (rhGH) Somatotropin Method validation LC-MS MS Pharmacokinetics |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2022.115188 |
| 通讯作者 | Yu, Jing-hua(yujinghua2@simm.ac.cn) ; Diao, Xing-xing(xxdiao@simm.ac.cn) |
| 英文摘要 | Recombinant human growth hormone (rhGH) is a peptide comprising 191 amino acids, that is mainly used to promote the growth of children and plays an important antiaging role. In the present study, a simple and sen-sitive quantitation method for rhGH in rat plasma was established by LC-MS/MS. After simple and rapid enzymatic digestion of the plasma sample, two suitable surrogate peptides (LFDNAMLR and FPTIPLSR) were selected for quantitative analysis. The results showed good linearity over calibration range 10-2000 ng/mL. The quality control (QC) accuracy ranged from-13.8 to 14.3%, and the accuracy of the lower limit of quantification (LLOQ) ranged from-12.9 to 19.0%. The intra-day and inter-day precision ranges for all QCs were 1.7-13.6% and 4.0-7.0%, respectively. The method was successfully applied to intravenous and subcutaneous pharmaco-kinetic studies in rats. In comparison with previously published methods, our method features simple sample preparation combined with a short sample processing time (3.5 h), wide linear range (10-2000 ng/mL), small plasma volume (35 mu L), and LLOQ (10 ng/mL). |
| WOS关键词 | MASS-SPECTROMETRY ; SENSITIVE ELISA ; QUANTIFICATION ; ASSAY ; SERUM ; PEPTIDES ; ANTIBODY |
| 资助项目 | National Natural Science Foundation of China ; [81903701] ; [82104276] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000908053300003 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/303896]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yu, Jing-hua; Diao, Xing-xing |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wen-si,Yu, Jing-hua,Diao, Xing-xing. Development and validation of a LC-MS/MS method for quantitation of recombinant human growth hormone in rat plasma and application to a pharmacokinetic study[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2023,224:9. |
| APA | Huang, Wen-si,Yu, Jing-hua,&Diao, Xing-xing.(2023).Development and validation of a LC-MS/MS method for quantitation of recombinant human growth hormone in rat plasma and application to a pharmacokinetic study.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,224,9. |
| MLA | Huang, Wen-si,et al."Development and validation of a LC-MS/MS method for quantitation of recombinant human growth hormone in rat plasma and application to a pharmacokinetic study".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 224(2023):9. |
入库方式： OAI收割
来源：上海药物研究所
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