Generation of an Aptamer Targeting Receptor-Type Tyrosine-Protein Phosphatase F
文献类型：期刊论文
| 作者 | Lv, Jing1,2; Zhen, Xiaoxiao1,2; Li, Dandan1,2; Liao, Guomiao3,4; Long, Zhenhao1,5; Zhang, Nan1; Liu, Xiangjun1,5; Bing, Tao1,3,6; Shangguan, Dihua1,3,5 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2023-01-03 |
| 页码 | 6 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.2c03988 |
| 通讯作者 | Bing, Tao(bingtao@iccas.ac.cn) ; Shangguan, Dihua(sgdh@iccas.ac.cn) |
| 英文摘要 | Cell-SELEX is a powerful tool to generate aptamers that specifically bind the native molecules on living cells. Here, we report an aptamer ZAJ4a generated by cell-SELEX. The molecular target of ZAJ4a was pulled down by the enriched cell-SELEX pool and identified to be the receptor-type tyrosine-protein phosphatase F (PTPRF) through a stable isotope labeling using amino acids in cell culture (SILAC)-based quantitative proteomic method. ZAJ4a showed high binding affinity with nanomolar range to cancer cells expressing PTPRF. Meanwhile, PTPRF was proven to highly express on several cancer cell lines using ZAJ4a as a molecular probe and to highly express in many kinds of cancer samples using gene expression profiling interactive analysis (GEPIA2) from the TCGA and GTEx databases. These results indicate that the aptamer generated by cell-SELEX showed good specificity at the molecular level. This cell-SELEX and target identification strategies show great potential for identifying biomarkers on the cell surface. |
| WOS关键词 | CELL ; DISCOVERY ; DNA |
| 资助项目 | National Natural Science Foundation of China ; [21874140] ; [21635008] ; [22174149] ; [22274139] ; [21877115] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000908462200001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/303923]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Bing, Tao; Shangguan, Dihua |
| 作者单位 | 1.Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Inst Chem, Beijing Natl Lab Mol Sci,Key Lab Analyt Chem Livi, Beijing 100190, Peoples R China 2.China Univ Petr, Coll New Energy & Mat, Beijing 102249, Peoples R China 3.Univ Chinese Acad Sci, Sch Mol Med, Hangzhou Inst Adv Study, Hangzhou 310013, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, Sch Chem Sci, Beijing 100049, Peoples R China 6.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Key Lab Zhejiang Prov Aptamers & Theranost, Hangzhou 310022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Jing,Zhen, Xiaoxiao,Li, Dandan,et al. Generation of an Aptamer Targeting Receptor-Type Tyrosine-Protein Phosphatase F[J]. ANALYTICAL CHEMISTRY,2023:6. |
| APA | Lv, Jing.,Zhen, Xiaoxiao.,Li, Dandan.,Liao, Guomiao.,Long, Zhenhao.,...&Shangguan, Dihua.(2023).Generation of an Aptamer Targeting Receptor-Type Tyrosine-Protein Phosphatase F.ANALYTICAL CHEMISTRY,6. |
| MLA | Lv, Jing,et al."Generation of an Aptamer Targeting Receptor-Type Tyrosine-Protein Phosphatase F".ANALYTICAL CHEMISTRY (2023):6. |
入库方式： OAI收割
来源：上海药物研究所
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