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The Aurora Kinase Inhibitor CYC116 Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells

文献类型：期刊论文

作者Ji, Sijia2,3,4; Tu, Wanzhi2,3,4; Huang, Chenwen3; Chen, Ziyang3,4; Ren, Xinyue3,4; He, Bingqing2,3,4; Ding, Xiaoyan1; Chen, Yuelei1; Xie, Xin2,3,4,5
刊名MOLECULES AND CELLS
出版日期2022-12-01
卷号45期号:12页码:923-934
关键词Aurora kinase inhibitor cardiomyocyte differen-tiation cell cycle human pluripotent stem cell maturation
ISSN号1016-8478
DOI10.14348/molcells.2022.0077
通讯作者Xie, Xin(xxie@simm.ac.cn)
英文摘要Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have great potential in applications such as regenerative medicine, cardiac disease modeling, and in vitro drug evaluation. However, hPSC-CMs are immature, which limits their applications. During development, the maturation of CMs is accompanied by a decline in their proliferative capacity. This phenomenon suggests that regulating the cell cycle may facilitate the maturation of hPSC-CMs. Aurora kinases are essential kinases that regulate the cell cycle, the role of which is not well studied in hPSC-CM maturation. Here, we demonstrate that CYC116, an inhibitor of Aurora kinases, significantly promotes the maturation of CMs derived from both human embryonic stem cells (H1 and H9) and iPSCs (induced PSCs) (UC013), resulting in increased expression of genes related to cardiomyocyte function, better organization of the sarcomere, increased sarcomere length, increased number of mitochondria, and enhanced physiological function of the cells. In addition, a number of other Aurora kinase inhibitors have also been found to promote the maturation of hPSC-CMs. Our data suggest that blocking aurora kinase activity and regulating cell cycle progression may promote the maturation of hPSC-CMs.
WOS关键词FUNCTIONAL MATURATION ; METABOLIC MATURATION ; PROLIFERATION ; CONTRIBUTES ; GENERATION ; REVEALS ; PROFILE ; HEART
资助项目Chinese Acade-my of Sciences ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; [XDA16010202] ; [2017YFA0104002] ; [82121005] ; [81730099]
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
语种英语
WOS记录号WOS:000906898100001
出版者KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
源URL[http://119.78.100.183/handle/2S10ELR8/304031]  
专题新药研究国家重点实验室
通讯作者Xie, Xin
作者单位1.Chinese Acad Sci, Inst Biochem & Cell Biol, Stem Cell Bank Stem Cell Core Facil, Shanghai 200031, Peoples R China
2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
推荐引用方式
GB/T 7714		 Ji, Sijia,Tu, Wanzhi,Huang, Chenwen,et al. The Aurora Kinase Inhibitor CYC116 Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells[J]. MOLECULES AND CELLS,2022,45(12):923-934.
APA Ji, Sijia.,Tu, Wanzhi.,Huang, Chenwen.,Chen, Ziyang.,Ren, Xinyue.,...&Xie, Xin.(2022).The Aurora Kinase Inhibitor CYC116 Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells.MOLECULES AND CELLS,45(12),923-934.
MLA Ji, Sijia,et al."The Aurora Kinase Inhibitor CYC116 Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells".MOLECULES AND CELLS 45.12(2022):923-934.

入库方式： OAI收割

来源：上海药物研究所

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