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Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice

文献类型：期刊论文

作者Chen, Yexiang1,2,3; Yao, Song-Yu2,3,4; Shu, Xuelian2,3; Wang, Yu-Jun2,3; Liu, Jing-Gen1,2,3
刊名BEHAVIOURAL BRAIN RESEARCH
出版日期2023-02-13
卷号438页码:10
关键词Chronic social defeat stress Prelimbic cortex MRNA sequencing MiRNA sequencing GPR35 signaling
ISSN号0166-4328
DOI10.1016/j.bbr.2022.114211
通讯作者Wang, Yu-Jun(yjwang@simm.ac.cn) ; Liu, Jing-Gen(jgliu@simm.ac.cn)
英文摘要Major depressive disorder is a complex psychiatric disorder with a high prevalence rate worldwide. Previous studies have demonstrated the involvement of the prelimbic cortex (PL) in mediating depressive-like behavior, however, the exact molecular mechanism taking place in the PL remains unclear. In the present study, we conducted high-throughput sequencing of mRNAs and miRNAs in PL tissue harvested from chronic social defeat stress (CSDS) susceptible male mice. We identified 59 differentially expressed mRNAs and 6 differentially expressed miRNAs, in which 40 mRNAs and 3 miRNAs were up-regulated, while 19 mRNAs and 3 miRNAs were down-regulated. Integrated analysis of miRNA-mRNA networks suggested that GPR35 signaling might be involved in CSDS-induced depressive-like behaviors. RT-PCR and western blot assays validated that Abra, Sell and GPR35 were up-regulated. Functionally, inhibition of GPR35 in the PL ameliorated CSDS-induced depressive-like behaviors. Thus, the present study provided a global view of mRNA and miRNA profiles in the PL of male stress susceptible mice, and suggested that GPR35 signaling was associated with CSDS-induced depressivelike behaviors. These results may be valuable for further investigations of the molecular regulatory mechanisms in stress-induced depression.
WOS关键词MEDIAL PREFRONTAL CORTEX ; GENE-EXPRESSION ; BEHAVIOR ; RECEPTOR ; RAT ; SUSCEPTIBILITY ; GPR35
资助项目Ministry of Science and Technology[2021ZD0202900] ; Ministry of Science and Technology[2021ZD0203500] ; National Natural Science Foundation of China[82030112] ; National Natural Science Foundation of China[81773710] ; Science and Technology Commission of Shanghai Municipality[20ZR1468200] ; Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions[NYKFKT2019015]
WOS研究方向Behavioral Sciences ; Neurosciences & Neurology
语种英语
WOS记录号WOS:000897721300005
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/304159]  
专题新药研究国家重点实验室
通讯作者Wang, Yu-Jun; Liu, Jing-Gen
作者单位1.China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing 210009, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Yexiang,Yao, Song-Yu,Shu, Xuelian,et al. Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice[J]. BEHAVIOURAL BRAIN RESEARCH,2023,438:10.
APA Chen, Yexiang,Yao, Song-Yu,Shu, Xuelian,Wang, Yu-Jun,&Liu, Jing-Gen.(2023).Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice.BEHAVIOURAL BRAIN RESEARCH,438,10.
MLA Chen, Yexiang,et al."Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice".BEHAVIOURAL BRAIN RESEARCH 438(2023):10.

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来源：上海药物研究所

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