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IL6 supports long-term expansion of hepatocytes in vitro

文献类型：期刊论文

作者Guo, Ren1; Jiang, Mengmeng1,2,3; Wang, Gang4; Li, Bing1,3,5; Jia, Xiaohui1,3; Ai, Yan1,3,5; Chen, Shanshan1,3; Tang, Peilan1,6; Liu, Aijie1; Yuan, Qianting1
刊名NATURE COMMUNICATIONS
出版日期2022-11-29
卷号13期号:1页码:13
DOI10.1038/s41467-022-35167-8
通讯作者Xie, Xin(xxie@simm.ac.cn)
英文摘要Hepatocytes are very difficult to expand in vitro. A few studies have demonstrated that chemical cocktails with growth factors or Wnt ligands can support long-term expansion of hepatocytes via dedifferentiation. However, the culture conditions are complex, and clonal expansion of hepatic progenitors with full differentiation capacity are rarely reported. Here, we discover IL6, combined with EGF and HGF, promotes long-term expansion (>30 passages in similar to 150 days with theoretical expansion of similar to 10(35) times) of primary mouse hepatocytes in vitro in simple 2D culture, by converting hepatocytes into induced hepatic progenitor cells (iHPCs), which maintain the capacity of differentiation into hepatocytes. IL6 also supports the establishment of single hepatocyte-derived iHPC clones. The summation of the downstream STAT3, ERK and AKT pathways induces a number of transcription factors which support rapid growth. This physiological and simple way may provide ideas for culturing previously difficult-to-culture cell types and support their future applications.
WOS关键词LIVER-REGENERATION ; HEPATIC-DYSFUNCTION ; MOUSE FIBROBLASTS ; PROGENITOR CELLS ; MURINE MODEL ; NECROSIS ; GROWTH ; EXPRESSION ; PROMOTES ; BARX2
WOS研究方向Science & Technology - Other Topics
语种英语
出版者NATURE PORTFOLIO
WOS记录号WOS:000890398000017
源URL[http://119.78.100.183/handle/2S10ELR8/304306]  
专题新药研究国家重点实验室
通讯作者Xie, Xin
作者单位1.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Fudan Univ, Sch Pharm, Dept Pharmaceut, Shanghai 201203, Peoples R China
5.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China
6.Nanchang Univ, Sch Pharmaceut Sci, Nanchang 330006, Jiangxi, Peoples R China
7.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Ren,Jiang, Mengmeng,Wang, Gang,et al. IL6 supports long-term expansion of hepatocytes in vitro[J]. NATURE COMMUNICATIONS,2022,13(1):13.
APA Guo, Ren.,Jiang, Mengmeng.,Wang, Gang.,Li, Bing.,Jia, Xiaohui.,...&Xie, Xin.(2022).IL6 supports long-term expansion of hepatocytes in vitro.NATURE COMMUNICATIONS,13(1),13.
MLA Guo, Ren,et al."IL6 supports long-term expansion of hepatocytes in vitro".NATURE COMMUNICATIONS 13.1(2022):13.

入库方式： OAI收割

来源：上海药物研究所

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