PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling
文献类型：期刊论文
| 作者 | Zhai, Linhui2,3,4; Wang, Le2,4; Hu, Hao4; Liu, Quan4; Lee, Sangkyu1,5; Tan, Minjia2,4 ; Zhang, Yinan2,3
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| 刊名 | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
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| 出版日期 | 2022-08-31 |
| 卷号 | 10页码:13 |
| ISSN号 | 2296-634X |
| 关键词 | C-terminomics chemical derivatization enrichment high-efficiency post-translation modification (PTM) |
| DOI | 10.3389/fcell.2022.995590 |
| 通讯作者 | Tan, Minjia(mjtan@simm.ac.cn) ; Zhang, Yinan(yinanzhang@njucm.edu.cn) |
| 英文摘要 | High-throughput profiling of protein C-termini is still a challenging task. Proteomics provides a powerful technology for systematic and high-throughput study of protein C-termini. Various C-terminal peptide enrichment strategies based on chemical derivatization and chromatography separation have been reported. However, they are still costly and time-consuming, with low enrichment efficiency for C-terminal peptides. In this study, by taking advantage of the high reaction selectivity of 2-pyridinecarboxaldehyde (2-PCA) with an alpha-amino group on peptide N-terminus and high affinity between biotin and streptavidin, we developed a 2-PCA- and biotin labeling-based C-terminomic (PBC) strategy for a high-efficiency and high-throughput analysis of protein C-terminome. Triplicates of PBC experiments identified a total of 1,975 C-terminal peptides corresponding to 1,190 proteins from 293 T cell line, which is 180% higher than the highest reported number of C-terminal peptides identified from mammalian cells by chemical derivatization-based C-terminomics study. The enrichment efficiency (68%) is the highest among the C-terminomics methods currently reported. In addition, we not only uncovered 50 proteins with truncated C-termini which were significantly enriched in extracellular exosome, vesicle, and ribosome by a bioinformatic analysis but also systematically characterized the whole PTMs on C-terminal in 293 T cells, suggesting PBC as a powerful tool for protein C-terminal degradomics and PTMs investigation. In conclusion, the PBC strategy would benefit high-efficiency and high-throughput profiling of protein C-terminome. |
| WOS关键词 | PROTEOMICS ; PROTEASES ; ROLES |
| 资助项目 | National Key R and D Program of China[2020YFE0202200] ; Natural Science Foundation of China[32071432] ; Natural Science Foundation of China[21877062] ; Natural Science Foundation of China[32171434] ; Basic research projects of Shanghai Science and Technology Commission science and technology innovation action plan[19JC1416300] ; open fund of state key laboratory of Pharmaceutical Biotechnology, Nanjing University, China[KF-202201] ; NSFC-NRF (China-Korea) Joint Research Program[82111540276] |
| WOS研究方向 | Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000891528600001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/304389]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Tan, Minjia; Zhang, Yinan |
| 作者单位 | 1.Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu, South Korea 2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Sch Pharm, Nanjing, Jiangsu, Peoples R China 3.Nanjing Univ Chinese Med, Sch Pharm, Jiangsu Key Lab Funct Subst Chinese Med, Nanjing, Jiangsu, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 5.Kyungpook Natl Univ, Coll Pharm, Daegu, South Korea |
| 推荐引用方式 GB/T 7714 | Zhai, Linhui,Wang, Le,Hu, Hao,et al. PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2022,10:13. |
| APA | Zhai, Linhui.,Wang, Le.,Hu, Hao.,Liu, Quan.,Lee, Sangkyu.,...&Zhang, Yinan.(2022).PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,10,13. |
| MLA | Zhai, Linhui,et al."PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10(2022):13. |
入库方式： OAI收割
来源：上海药物研究所
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