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Neuroprotective effects of a lead compound from coral via modulation of the orphan nuclear receptor Nurr1

文献类型：期刊论文

作者Su, Jian-Wei1; Yang, Pei1; Xing, Mei-Mei1; Chen, Bao2; Xie, Xia-Hong1; Ding, Jian-Hua; Lu, Ming3; Liu, Yang1,4; Guo, Yue-Wei2,5; Hu, Gang1,4
刊名CNS NEUROSCIENCE & THERAPEUTICS
出版日期2022-11-23
页码14
ISSN号1755-5930
关键词C elegans corals-derived compounds neuroprotection Nurr1 SH-SY5Y cells
DOI10.1111/cns.14025
通讯作者Liu, Yang(liuyang@njmu.edu.cn) ; Guo, Yue-Wei(ywguo@simm.ac.cn) ; Hu, Gang(neuropha@njmu.edu.cn)
英文摘要AimsTo screen coral-derived compounds with neuroprotective activity and clarify the potential mechanism of lead compounds. MethodsThe lead compounds with neuroprotective effects were screened by H2O2 and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPP+)-induced cell damage models in SH-SY5Y cells. CCK8 and LDH assays were used to detect cell viability. The anti-apoptosis of lead compounds was evaluated by flow cytometry. JC-1 and MitoSox assays were performed to examine the changes in mitochondrial membrane potential and mitochondrial ROS level. Survival of primary cortical and dopaminergic midbrain neurons was measured by MAP2 and TH immunoreactivities. The Caenorhabditis elegans (C. elegans) model was established to determine the effect of lead compounds on dopaminergic neurons and behavior changes. ResultsThree compounds (No. 63, 68, and 74), derived from marine corals, could markedly alleviate the cell damage and notably reverse the loss of worm dopaminergic neurons. Further investigation indicated that compound 63 could promote the expression of Nurr1 and inhibit neuronal apoptosis signaling pathways. ConclusionLead compounds from marine corals exerted significant neuroprotective effects? which indicated that coral might be a new and potential resource for screening and isolating novel natural compounds with neuroprotective effects. Furthermore, this study also provided a new strategy for the clinical treatment of neurodegenerative diseases such as Parkinson's disease.
WOS关键词PARKINSONS-DISEASE ; CAENORHABDITIS-ELEGANS ; MODEL ; METABOLITES ; EXPRESSION
资助项目National Key R&D Program of China ; National Natural Science Foundation of China ; [2021ZD0202901] ; [81991523] ; [82003725]
WOS研究方向Neurosciences & Neurology ; Pharmacology & Pharmacy
语种英语
出版者WILEY
WOS记录号WOS:000889227800001
源URL[http://119.78.100.183/handle/2S10ELR8/304545]  
专题新药研究国家重点实验室
通讯作者Liu, Yang; Guo, Yue-Wei; Hu, Gang
作者单位1.Nanjing Univ Chinese Med, Sch Med & Holist Integrat Med, Dept Pharmacol, Nanjing, Jiangsu, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
3.Nanjing Med Univ, Dept Pharmacol, Jiangsu Key Lab Neurodegenerat, Nanjing, Peoples R China
4.Nanjing Univ Chinese Med, Dept Pharmacol, 138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Zu Chong Zhi Rd 555, Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Su, Jian-Wei,Yang, Pei,Xing, Mei-Mei,et al. Neuroprotective effects of a lead compound from coral via modulation of the orphan nuclear receptor Nurr1[J]. CNS NEUROSCIENCE & THERAPEUTICS,2022:14.
APA Su, Jian-Wei.,Yang, Pei.,Xing, Mei-Mei.,Chen, Bao.,Xie, Xia-Hong.,...&Hu, Gang.(2022).Neuroprotective effects of a lead compound from coral via modulation of the orphan nuclear receptor Nurr1.CNS NEUROSCIENCE & THERAPEUTICS,14.
MLA Su, Jian-Wei,et al."Neuroprotective effects of a lead compound from coral via modulation of the orphan nuclear receptor Nurr1".CNS NEUROSCIENCE & THERAPEUTICS (2022):14.

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来源：上海药物研究所

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