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Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor

文献类型：期刊论文

作者Zhao, Li-Hua2,6; Lin, Jingyu3; Ji, Su-Yu4; Zhou, X. Edward5; Mao, Chunyou4; Shen, Dan-Dan4; He, Xinheng2,6; Xiao, Peng3; Sun, Jinpeng3; Melcher, Karsten5
刊名NATURE COMMUNICATIONS
出版日期2022-11-05
卷号13期号:1页码:13
DOI10.1038/s41467-022-33851-3
通讯作者Zhao, Li-Hua(zhaolihuawendy@simm.ac.cn) ; Zhang, Yan(zhang_yan@zju.edu.cn) ; Yu, Xiao(yuxiao@sdu.edu.cn) ; Xu, H. Eric(eric.xu@simm.ac.cn)
英文摘要The ability to couple with multiple G protein subtypes, such as G(s), G(i/o), or G(q/11), by a given G protein-coupled receptor (GPCR) is critical for many physiological processes. Over the past few years, the cryo-EM structures for all 15 members of the medically important class B GPCRs, all in complex with G(s) protein, have been determined. However, no structure of class B GPCRs with G(q/11) has been solved to date, limiting our understanding of the precise mechanisms of G protein coupling selectivity. Here we report the structures of corticotropin releasing factor receptor 2 (CRF2R) bound to Urocortin 1 (UCN1), coupled with different classes of heterotrimeric G proteins, G(11) and G(o). We compare these structures with the structure of CRF2R in complex with G(s) to uncover the structural differences that determine the selective coupling of G protein subtypes by CRF2R. These results provide important insights into the structural basis for the ability of CRF2R to couple with multiple G protein subtypes. Here, the authors report structures of corticotropin releasing factor receptor 2 (CRF2R) bound to agonist Urocortin 1 (UCN1) and coupled to G proteins G(11) and G(o), offering insight into the structural basis for the ability of CRF2R to couple with multiple G protein subtypes.
WOS关键词CRYO-EM STRUCTURE ; SIGNAL-TRANSDUCTION PATHWAY ; BIOLOGICAL-ACTIVITY ; HORMONE-RECEPTORS ; GLP-1 RECEPTOR ; KINASE ; FAMILY ; PEPTIDE ; SPECIFICITY ; RECOGNITION
资助项目National Natural Science Foundation of China[32071203] ; National Natural Science Foundation of China[31971195] ; National Natural Science Foundation of China[81922071] ; National Natural Science Foundation of China[2100959] ; National Key R&D Program of China[2019YFA0904200] ; Young Innovator Association of CAS[2018325] ; SA-SIBS Scholarship Program ; Ministry of Science and Technology (China)[2018YFA0507002] ; Shanghai Municipal Science and TechnologyMajor Project[2019SHZDZX02] ; Shanghai Municipal Science and TechnologyMajor Project[18ZR1447800] ; CAS Strategic Priority Research Program[XDB08020303] ; Zhejiang Province Science Fund for Distinguished Young Scholars[LR19H310001] ; Key R&D Projects of Zhejiang Province[2021C03039] ; MOE Frontier Science Center for Brain Science & Brain-Machine Integration, Zhejiang University
WOS研究方向Science & Technology - Other Topics
语种英语
出版者NATURE PORTFOLIO
WOS记录号WOS:000879737500003
源URL[http://119.78.100.183/handle/2S10ELR8/304566]  
专题中国科学院上海药物研究所
通讯作者Zhao, Li-Hua; Zhang, Yan; Yu, Xiao; Xu, H. Eric
作者单位1.Zhejiang Prov Key Lab Immun & Inflammatory Dis, Hangzhou 310058, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shandong Univ, Sch Basic Med Sci, Dept Physiol, Jinan 250012, Peoples R China
4.Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Biophys & Pathol, Hangzhou 310058, Peoples R China
5.Van Andel Res Inst, Dept Struct Biol, Grand Rapids, MI 49503 USA
6.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
7.Zhejiang Univ, Liangzhu Lab, Med Ctr, Hangzhou 311121, Peoples R China
8.Zhejiang Univ, MOE Frontier Sci Ctr Brain Res & Brain Machine In, Sch Med, Hangzhou 310058, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Li-Hua,Lin, Jingyu,Ji, Su-Yu,et al. Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor[J]. NATURE COMMUNICATIONS,2022,13(1):13.
APA Zhao, Li-Hua.,Lin, Jingyu.,Ji, Su-Yu.,Zhou, X. Edward.,Mao, Chunyou.,...&Xu, H. Eric.(2022).Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor.NATURE COMMUNICATIONS,13(1),13.
MLA Zhao, Li-Hua,et al."Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor".NATURE COMMUNICATIONS 13.1(2022):13.

入库方式： OAI收割

来源：上海药物研究所

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