Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells
文献类型：期刊论文
| 作者 | Cao, Jialing5; Li HY(李航宇); Tang, Hongyan; Gu, Xuenan; Wang, Yan2; Guan DS(关东石); Du, Jing; Fan, Yubo |
| 刊名 | BIOENGINEERING BASEL
 |
| 出版日期 | 2023-03 |
| 卷号 | 10期号:3页码:384 |
| 关键词 | embryo implantation human choriocarcinoma cell extracellular matrix stiffness durotaxis |
| DOI | 10.3390/bioengineering10030384 |
| 英文摘要 | The effect of extracellular matrix (ECM) stiffness on embryonic trophoblast cells invasion during mammalian embryo implantation remains largely unknown. In this study, we investigated the effects of ECM stiffness on various aspects of human trophoblast cell behaviors during cell ECM interactions. The mechanical microenvironment of the uterus was simulated by fabricating polyacrylamide (PA) hydrogels with different levels of stiffness. The human choriocarcinoma (JAR) cell lineage was used as the trophoblast model. We found that the spreading area of JAR cells, the formation of focal adhesions, and the polymerization of the F actin cytoskeleton were all facilitated with increased ECM stiffness. Significantly, JAR cells also exhibited durotactic behavior on ECM with a gradient stiffness. Meanwhile, stiffness of the ECM affects the invasion of multicellular JAR spheroids. These results demonstrated that human trophoblast cells are mechanically sensitive, while the mechanical properties of the uterine microenvironment could play an important role in the implantation process. |
| 分类号 | 二类 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering, Biomedical |
| 语种 | 英语 |
| WOS记录号 | WOS:000957330500001 |
| 资助机构 | National Natural Science Foundation of China [12222201, 82273500, U20A20390, 11827803, 11972351] ; Fundamental Research Funds for the Central Universities [ZG140S1971] ; National Key R&D Program of China [2021YFA0719302] ; Peking University Health Science Center X Cross Seed Fund [BMU2020MX014] ; Incubation fund for reserve candidates of Peking University Third Hospital [BYSYFY2021007] |
| 其他责任者 | Du, J ; Fan, YB (corresponding author), Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China. ; Guan, DS (corresponding author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China. ; Guan, DS (corresponding author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China. ; Wang, Y (corresponding author), Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China. |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/91791]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 作者单位 | 1.Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China 4.Beihang Univ, Sino French Engineer Sch, Beijing 100083, Peoples R China 5.Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Jialing,Li HY,Tang, Hongyan,et al. Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells[J]. BIOENGINEERING BASEL,2023,10(3):384. |
| APA | Cao, Jialing.,李航宇.,Tang, Hongyan.,Gu, Xuenan.,Wang, Yan.,...&Fan, Yubo.(2023).Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells.BIOENGINEERING BASEL,10(3),384. |
| MLA | Cao, Jialing,et al."Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells".BIOENGINEERING BASEL 10.3(2023):384. |
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来源：力学研究所
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