Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor
文献类型：期刊论文
| 作者 | Liu HY(刘浩雨); Jin G(靳刚)
|
| 刊名 | THIN SOLID FILMS
 |
| 出版日期 | 2023-03-31 |
| 卷号 | 769页码:139738 |
| ISSN号 | 0040-6090 |
| 关键词 | Aminosilane Surface modification Molecular orientation angle Specificity Immune globulin Imaging ellipsometry biosensor Sum frequency vibration spectrum |
| DOI | 10.1016/j.tsf.2023.139738 |
| 英文摘要 | 3 Aminopropyltriethoxysilane (APTES) has been widely used in biosensing as a surface modifying molecule that acts as a bridge. The orientation and structure of APTES at the interface are major factors that affect biosensing efficiency. IgG (immunoglobulin G) is the most used and one of the most abundant immune proteins in the serum. We not only used broadband sum frequency generation vibrational spectroscopy to compare the spectrum of APTES and 11 aminoundecyltriethoxysilane (AUTES) molecules modified on a Si (111) surface but also compared the number and sensitivity of IgG adhered on APTES and AUTES surfaces by the imaging ellipsometry biosensor. We first found that the terminal amino groups of the APTES molecule were disorderly arranged by Sum frequency vibration spectrum (SFVS). Then we compared the performance of IgG immobilization in APTES or AUTES surfaces by imaging ellipsometry biosensor (IEB). In IEB, the anti IgG detection LOD (limit of detec tion) on the APTES modified surface(51 ng/mL) was higher than that on the AUTES modified surface(380 ng/ mL). We explored the relationship between the stability of aminosilane and the stability of biomolecules, and provided a reference for the selection of surface modification methods using aminosilane as a self assembled membrane. |
| 分类号 | Q3 |
| WOS研究方向 | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000948432900001 |
| 其他责任者 | Jin, G (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/91816]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Fundamental Phys & Math Sci, 1 Sub Lane Xiangshan, Hangzhou 310024, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu HY,Jin G. Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor[J]. THIN SOLID FILMS,2023,769:139738. |
| APA | 刘浩雨,&靳刚.(2023).Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor.THIN SOLID FILMS,769,139738. |
| MLA | 刘浩雨,et al."Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor".THIN SOLID FILMS 769(2023):139738. |
入库方式： OAI收割
来源：力学研究所
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