Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry
文献类型：期刊论文
| 作者 | Wang CY(王晨昱); Liu W(刘巍) ; Niu Y(牛宇); Xu P(徐鹏); Jin G(靳刚)
|
| 刊名 | THIN SOLID FILMS
 |
| 出版日期 | 2022-12-01 |
| 卷号 | 763页码:139578 |
| 关键词 | Imaging ellipsometry Biosensor Quantitative detection |
| ISSN号 | 0040-6090 |
| DOI | 10.1016/j.tsf.2022.139578 |
| 英文摘要 | The biosensor based on imaging ellipsometry (BIE) is a practical technique to measure protein interactions such as the immobilization of biomolecules and the recognition between ligand and analyte. In this paper, to avoid the working condition optimization before each measurement, a fixed linear working condition for the silicon wafer chip is established at the angle of incidence of 75 degrees with the fixed azimuths of the polarizer and the analyzer P = 90 degrees and A = 45 degrees. Further, a quantitative model is constructed to establish the standard curve between the ellipsometric signal and the protein concentration. As a result, this model is used to deduce the dissociation equilibrium constants of three relevant blood proteins as well as their antibodies. This work bridges the gap between the biochemical demand and BIE for sensing applications. |
| 分类号 | Q3 |
| WOS研究方向 | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000950649200001 |
| 资助机构 | National Natural Science Foundation of China [81872584] |
| 其他责任者 | Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. ; Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/91817]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Foundamenal Phys & Math Sci, Hangzhou 310024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang CY,Liu W,Niu Y,et al. Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry[J]. THIN SOLID FILMS,2022,763:139578. |
| APA | 王晨昱,刘巍,牛宇,徐鹏,&靳刚.(2022).Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry.THIN SOLID FILMS,763,139578. |
| MLA | 王晨昱,et al."Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry".THIN SOLID FILMS 763(2022):139578. |
入库方式： OAI收割
来源：力学研究所
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