Sequencing Angiosperm Plastid Genomes Made Easy: A Complete Set of Universal Primers and a Case Study on the Phylogeny of Saxifragales
文献类型：期刊论文
| 作者 | Dong, Wenpan; Xu, Chao2; Cheng, Tao2; Lin, Kui1; Zhou, Shiliang |
| 刊名 | GENOME BIOLOGY AND EVOLUTION
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:5页码:989-997 |
| 关键词 | plastid genome universal primers Saxifragales |
| ISSN号 | 1759-6653 |
| DOI | 10.1093/gbe/evt063 |
| 文献子类 | Article |
| 英文摘要 | Plastid genomes are an invaluable resource for plant biological studies. However, the number of completely sequenced plant plastid genomes is still small compared with the vast number of species. To provide an alternative generalized approach, we designed a set of 138 pairs of universal primers for amplifying (termed short-range PCR) and sequencing the entire genomes of the angiosperm plastid genomes. The universality of the primers was tested by using species from the basal to asterid angiosperms. The polymerase chain reaction (PCR) success rate was higher than 96%. We sequenced the complete chloroplast genome of Liquidambar formosana as an example using this method and compared it to the genomes independently determined by long-range PCR (from 6.3 kb to 13.3 kb) and next-generation sequencing methods. The three genomes showed that they were completely identical. To test the phylogenetic efficiency of this method, we amplified and sequenced 18 chloroplast regions of 19 Saxifragales and Saxifragales related taxa, as a case study, to reconstruct the phylogeny of all families of the order. Phylograms based on a combination of our data, together with those from GenBank, clearly indicate three family groups and three single families within the order. This set of universal primers is expected to accelerate the accumulation of angiosperm plastid genomes and to make faster mass data collection of plastid genomes for molecular systematics. |
| 学科主题 | Evolutionary Biology ; Genetics & Heredity |
| 出版地 | OXFORD |
| WOS关键词 | COMPLETE CHLOROPLAST GENOME ; COMPLETE NUCLEOTIDE-SEQUENCE ; INVERTED REPEAT ; EVOLUTION ; INSIGHTS ; PINACEAE ; GENES ; DIVERSIFICATION ; IDENTIFICATION ; REARRANGEMENTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000324593500019 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Science and Technology(Ministry of Science, ICT & Future Planning, Republic of Korea) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27880]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 3.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Wenpan,Xu, Chao,Cheng, Tao,et al. Sequencing Angiosperm Plastid Genomes Made Easy: A Complete Set of Universal Primers and a Case Study on the Phylogeny of Saxifragales[J]. GENOME BIOLOGY AND EVOLUTION,2013,5(5):989-997. |
| APA | Dong, Wenpan,Xu, Chao,Cheng, Tao,Lin, Kui,&Zhou, Shiliang.(2013).Sequencing Angiosperm Plastid Genomes Made Easy: A Complete Set of Universal Primers and a Case Study on the Phylogeny of Saxifragales.GENOME BIOLOGY AND EVOLUTION,5(5),989-997. |
| MLA | Dong, Wenpan,et al."Sequencing Angiosperm Plastid Genomes Made Easy: A Complete Set of Universal Primers and a Case Study on the Phylogeny of Saxifragales".GENOME BIOLOGY AND EVOLUTION 5.5(2013):989-997. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。