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Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics

文献类型：期刊论文

作者Hu, Yuan-Yuan; Fan, Da-Yong; Losciale, Pasquale4,5; Chow, Wah Soon3; Zhang, Wang-Feng
刊名PHOTOSYNTHESIS RESEARCH
出版日期2013
卷号117期号:1-3页码:517-528
关键词Chlorophyll fluorescence P700 Photosystem II Photoinactivation of photosystem II Repair of photosystem II
ISSN号0166-8595
DOI10.1007/s11120-013-9822-5
文献子类Article
英文摘要Using radioactively labelled amino acids to investigate repair of photoinactivated photosystem II (PS II) gives only a relative rate of repair, while using chlorophyll fluorescence parameters yields a repair rate coefficient for an undefined, variable location within the leaf tissue. Here, we report on a whole-tissue determination of the rate coefficient of photoinactivation k (i) , and that of repair k (r) in cotton leaf discs. The method assays functional PS II via a P700 kinetics area associated with PS I, as induced by a single-turnover, saturating flash superimposed on continuous background far-red light. The P700 kinetics area, directly proportional to the oxygen yield per single-turnover, saturating flash, was used to obtain both k (i) and k (r) . The value of k (i) , directly proportional to irradiance, was slightly higher when CO2 diffusion into the abaxial surface (richer in stomata) was blocked by contact with water. The value of k (r) , sizable in darkness, changed in the light depending on which surface was blocked by contact with water. When the abaxial surface was blocked, k (r) first peaked at moderate irradiance and then decreased at high irradiance. When the adaxial surface was blocked, k (r) first increased at low irradiance, then plateaued, before increasing markedly at high irradiance. At the highest irradiance, k (r) differed by an order of magnitude between the two orientations, attributable to different extents of oxidative stress affecting repair (Nishiyama et al., EMBO J 20: 5587-5594, 2001). The method is a whole-tissue, convenient determination of the rate coefficient of photoinactivation k (i) and that of repair k (r) .
学科主题Plant Sciences
出版地DORDRECHT
电子版国际标准刊号1573-5079
WOS关键词PROTEIN-SYNTHESIS ; ELECTRON-FLUXES ; D1 PROTEIN ; PHOTOINHIBITION ; LIGHT ; LEAVES ; PHOTOSYNTHESIS ; DEGRADATION ; PSII ; PHOTODAMAGE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000326604900038
出版者SPRINGER
资助机构Australian Research Council(Australian Research Council) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Key Technology R and D Program of China(National Key Technology R&D Program) ; Chinese Academy of Sciences(Chinese Academy of Sciences)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27979]  
专题植被与环境变化国家重点实验室
作者单位1.Univ Bologna, Dipartimento Sci Agr, I-40127 Bologna, Italy
2.Australian Natl Univ, Res Sch Biol, Div Plant Sci, Canberra, ACT 0200, Australia
3.Shihezi Univ, Xinjiang Prod & Construct Grp, Key Lab Oasis Ecoagr, Shihezi 832003, Peoples R China
4.Consiglio Ric Sperimentaz Agr, Res Unit Agr Dry Environm, I-70125 Bari, Italy
5.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Yuan-Yuan,Fan, Da-Yong,Losciale, Pasquale,et al. Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics[J]. PHOTOSYNTHESIS RESEARCH,2013,117(1-3):517-528.
APA Hu, Yuan-Yuan,Fan, Da-Yong,Losciale, Pasquale,Chow, Wah Soon,&Zhang, Wang-Feng.(2013).Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics.PHOTOSYNTHESIS RESEARCH,117(1-3),517-528.
MLA Hu, Yuan-Yuan,et al."Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics".PHOTOSYNTHESIS RESEARCH 117.1-3(2013):517-528.

入库方式： OAI收割

来源：植物研究所

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