Expression of a Medicago falcata small GTPase gene, MfARL1 enhanced tolerance to salt stress in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Wang, Tian-Zuo; Xia, Xiu-Zhi; Zhao, Min-Gui; Tian, Qiu-Ying; Zhang, Wen-Hao
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| 刊名 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 63页码:227-235 |
| 关键词 | MfARL1 Medicago falcata Arabidopsis thaliana Salt stress Small GTPases Na+ accumulation |
| ISSN号 | 0981-9428 |
| DOI | 10.1016/j.plaphy.2012.12.004 |
| 文献子类 | Article |
| 英文摘要 | To understand the role of small GTPases in response to abiotic stress, we isolated a gene encoding a small GTPase, designated MfARL1, from a subtracted cDNA library in Medicago falcata, a native legume species in semi-arid grassland in northern China. The function of MfARL1 in response to salt stress was studied by expressing MfARL1 in Arabidopsis. Wild-type (WT) and transgenic plants constitutively expressing MfARL1 showed comparable phenotype when grown under control conditions. Germination of seeds expressing MfARL1 was less suppressed by salt stress than that of WT seeds. Transgenic seedlings had higher survival rate than WT seedlings under salt stress, suggesting that expression of MfARL1 confers tolerance to salt stress. The physiological and molecular mechanisms underlying these phenomena were elucidated. Salt stress led to a significant decrease in chlorophyll contents in WT plants, but not in transgenic plants. Transgenic plants accumulated less amounts of H2O2 and malondialdehyde than their WT counterparts under salt stress, which can be accounted for by the higher catalase activities, lower activities of superoxide dismutase, and peroxidase in transgenic plants than in WT plants. Transgenic plants displayed lower Na+/K+ ratio due to less accumulation of Na+ than wild-type under salt stress conditions. The lower Na+/K+ ratio may result from less accumulation of Na+ due to reduced expression of AtHKT1 that encodes Na+ transporter in transgenic plants under salt stress. These findings demonstrate that MfARL1 encodes a novel stress-responsive small GTPase that is involved in tolerance to salt stress. (c) 2013 Elsevier Masson SAS. All rights reserved. |
| 学科主题 | Plant Sciences |
| 出版地 | ISSY-LES-MOULINEAUX |
| 电子版国际标准刊号 | 1873-2690 |
| WOS关键词 | DROUGHT ; PLANTS ; RICE ; OVEREXPRESSION ; TRUNCATULA ; RADIATION ; RESPONSES ; SALINITY ; ENCODES ; OSRAN2 |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000315011400029 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 资助机构 | State Key Laboratory of Vegetation and Environmental Change |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28092]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Zhao, Min-Gui 2.Tian, Qiu-Ying 3.Xia, Xiu-Zhi 4.Chinese Acad Sci, State Key Lab Vegetat & Environm Change, Inst Bot, Beijing 100093, Peoples R China 5.[Wang, Tian-Zuo |
| 推荐引用方式 GB/T 7714 | Wang, Tian-Zuo,Xia, Xiu-Zhi,Zhao, Min-Gui,et al. Expression of a Medicago falcata small GTPase gene, MfARL1 enhanced tolerance to salt stress in Arabidopsis thaliana[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2013,63:227-235. |
| APA | Wang, Tian-Zuo,Xia, Xiu-Zhi,Zhao, Min-Gui,Tian, Qiu-Ying,&Zhang, Wen-Hao.(2013).Expression of a Medicago falcata small GTPase gene, MfARL1 enhanced tolerance to salt stress in Arabidopsis thaliana.PLANT PHYSIOLOGY AND BIOCHEMISTRY,63,227-235. |
| MLA | Wang, Tian-Zuo,et al."Expression of a Medicago falcata small GTPase gene, MfARL1 enhanced tolerance to salt stress in Arabidopsis thaliana".PLANT PHYSIOLOGY AND BIOCHEMISTRY 63(2013):227-235. |
入库方式： OAI收割
来源：植物研究所
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