The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis
文献类型：期刊论文
| 作者 | Xu, Ting-Ting; Song, Xiu-Fen; Ren, Shi-Chao; Liu, Chun-Ming
|
| 刊名 | BMC PLANT BIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 13 |
| 关键词 | CLV3 Peptide cleavage Flanking sequence AA substitution Stem cell regulation |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/1471-2229-13-225 |
| 文献子类 | Article |
| 英文摘要 | Background: Although it is known that CLAVATA3 (CLV3) acts as 12-and/ or 13-amino acid (AA) secreted peptides to regulate the number of stem cells in shoot apical meristems (SAMs), how functional CLV3 peptides are generated and if any particular sequences are required for the processing remain largely unknown. Results: We developed a mass spectrometry (MS)-based in vitro assay to monitor the cleavage of heterologously produced CLV3 fusion protein. Through co-cultivation of the fusion protein with Arabidopsis seedlings, we identified two cleavage sites: the previously reported one before Arg70 and a new one before Met39. Using synthetic peptides together with MALDI-Tof-MS analyses, we demonstrated that the non-conserved 5-AA motifs flanking N-termini of the CLV3 and its orthologous CLE1 peptides were critical for their cleavages and optimal activities in vitro. We also found that substitutions of Leu69 by Ala in fusion protein and in synthetic peptide of CLV3 compromised their cleavages, leading to significantly reduced activities in regulating the sizes of shoot and root meristems. Conclusions: These results suggest that 5-AA residues flanking the N-terminus of CLV3 peptide are required for proper cleavages and optimal function in stem cell regulation. |
| 学科主题 | Plant Sciences |
| 出版地 | LONDON |
| WOS关键词 | SECRETORY PEPTIDE ; RECEPTOR KINASE ; SMALL PROTEIN ; GENE ENCODES ; GROWTH ; POLYPEPTIDE ; CLAVATA3 ; MERISTEM ; SIGNAL ; FATE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000329090000001 |
| 出版者 | BMC |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Science and Technology of China(Ministry of Science and Technology, China) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27781]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Ren, Shi-Chao 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Song, Xiu-Fen 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.[Xu, Ting-Ting |
| 推荐引用方式 GB/T 7714 | Xu, Ting-Ting,Song, Xiu-Fen,Ren, Shi-Chao,et al. The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis[J]. BMC PLANT BIOLOGY,2013,13. |
| APA | Xu, Ting-Ting,Song, Xiu-Fen,Ren, Shi-Chao,&Liu, Chun-Ming.(2013).The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis.BMC PLANT BIOLOGY,13. |
| MLA | Xu, Ting-Ting,et al."The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis".BMC PLANT BIOLOGY 13(2013). |
入库方式： OAI收割
来源：植物研究所
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