中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis

文献类型:期刊论文

作者Xu, Ting-Ting; Song, Xiu-Fen; Ren, Shi-Chao; Liu, Chun-Ming
刊名BMC PLANT BIOLOGY
出版日期2013
卷号13
关键词CLV3 Peptide cleavage Flanking sequence AA substitution Stem cell regulation
ISSN号1471-2229
DOI10.1186/1471-2229-13-225
文献子类Article
英文摘要Background: Although it is known that CLAVATA3 (CLV3) acts as 12-and/ or 13-amino acid (AA) secreted peptides to regulate the number of stem cells in shoot apical meristems (SAMs), how functional CLV3 peptides are generated and if any particular sequences are required for the processing remain largely unknown. Results: We developed a mass spectrometry (MS)-based in vitro assay to monitor the cleavage of heterologously produced CLV3 fusion protein. Through co-cultivation of the fusion protein with Arabidopsis seedlings, we identified two cleavage sites: the previously reported one before Arg70 and a new one before Met39. Using synthetic peptides together with MALDI-Tof-MS analyses, we demonstrated that the non-conserved 5-AA motifs flanking N-termini of the CLV3 and its orthologous CLE1 peptides were critical for their cleavages and optimal activities in vitro. We also found that substitutions of Leu69 by Ala in fusion protein and in synthetic peptide of CLV3 compromised their cleavages, leading to significantly reduced activities in regulating the sizes of shoot and root meristems. Conclusions: These results suggest that 5-AA residues flanking the N-terminus of CLV3 peptide are required for proper cleavages and optimal function in stem cell regulation.
学科主题Plant Sciences
出版地LONDON
WOS关键词SECRETORY PEPTIDE ; RECEPTOR KINASE ; SMALL PROTEIN ; GENE ENCODES ; GROWTH ; POLYPEPTIDE ; CLAVATA3 ; MERISTEM ; SIGNAL ; FATE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000329090000001
出版者BMC
资助机构National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Science and Technology of China(Ministry of Science and Technology, China)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27781]  
专题中科院植物分子生理学重点实验室
作者单位1.Ren, Shi-Chao
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Song, Xiu-Fen
4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
5.[Xu, Ting-Ting
推荐引用方式
GB/T 7714
Xu, Ting-Ting,Song, Xiu-Fen,Ren, Shi-Chao,et al. The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis[J]. BMC PLANT BIOLOGY,2013,13.
APA Xu, Ting-Ting,Song, Xiu-Fen,Ren, Shi-Chao,&Liu, Chun-Ming.(2013).The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis.BMC PLANT BIOLOGY,13.
MLA Xu, Ting-Ting,et al."The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis".BMC PLANT BIOLOGY 13(2013).

入库方式: OAI收割

来源:植物研究所

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