2, 6-dichlorobenzonitrile Causes Multiple Effects on Pollen Tube Growth beyond Altering Cellulose Synthesis in Pinus bungeana Zucc
文献类型：期刊论文
| 作者 | Hao, Huaiqing; Chen, Tong; Fan, Lusheng; Li, Ruili; Wang, Xiaohua |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:10 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0076660 |
| 文献子类 | Article |
| 英文摘要 | Cellulose is an important component of cell wall, yet its location and function in pollen tubes remain speculative. In this paper, we studied the role of cellulose synthesis in pollen tube elongation in Pinus bungeana Zucc. by using the specific inhibitor, 2, 6-dichlorobenzonitrile (DCB). In the presence of DCB, the growth rate and morphology of pollen tubes were distinctly changed. The organization of cytoskeleton and vesicle trafficking were also disturbed. Ultrastructure of pollen tubes treated with DCB was characterized by the loose tube wall and damaged organelles. DCB treatment induced distinct changes in tube wall components. Fluorescence labeling results showed that callose, and acidic pectin accumulated in the tip regions, whereas there was less cellulose when treated with DCB. These results were confirmed by FTIR microspectroscopic analysis. In summary, our findings showed that inhibition of cellulose synthesis by DCB affected the organization of cytoskeleton and vesicle trafficking in pollen tubes, and induced changes in the tube wall chemical composition in a dose-dependent manner. These results confirm that cellulose is involved in the establishment of growth direction of pollen tubes, and plays important role in the cell wall construction during pollen tube development despite its lower quantity. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | CELL-WALL ; CALLOSE SYNTHASE ; PLASMA-MEMBRANE ; ROOT HAIRS ; LOCALIZATION ; PECTIN ; ACTIN ; BIOSYNTHESIS ; 2,6-DICHLOROBENZONITRILE ; ACCUMULATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000325819400069 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27783]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Huaiqing,Chen, Tong,Fan, Lusheng,et al. 2, 6-dichlorobenzonitrile Causes Multiple Effects on Pollen Tube Growth beyond Altering Cellulose Synthesis in Pinus bungeana Zucc[J]. PLOS ONE,2013,8(10). |
| APA | Hao, Huaiqing,Chen, Tong,Fan, Lusheng,Li, Ruili,&Wang, Xiaohua.(2013).2, 6-dichlorobenzonitrile Causes Multiple Effects on Pollen Tube Growth beyond Altering Cellulose Synthesis in Pinus bungeana Zucc.PLOS ONE,8(10). |
| MLA | Hao, Huaiqing,et al."2, 6-dichlorobenzonitrile Causes Multiple Effects on Pollen Tube Growth beyond Altering Cellulose Synthesis in Pinus bungeana Zucc".PLOS ONE 8.10(2013). |
入库方式： OAI收割
来源：植物研究所
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