Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis
文献类型：期刊论文
| 作者 | Zhang, Dajian1; Wang, Xiaomin; Wang, Min; Li, Junhua; Guo, Xiaoyu; Chong, Kang; Xu, Yunyuan |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:9 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0075773 |
| 文献子类 | Article |
| 英文摘要 | WUSCHEL (WUS) is essential for preventing stem cell differentiation in Arabidopsis. Here we report that in addition to its functions in meristematic stem cell maintenance, WUS is involved in the regulation of cell division. The WUS gain-of-function mutant, stem ectopic flowers (sef), displayed elongated hypocotyls, whereas the loss-of-function wus-1 mutant had shortened hypocotyls. The long hypocotyl in sef was due to the presence of more cells, rather than increased cell elongation. Microscopic observation, flow cytometry assays, quantitative RT-PCR (qRT-PCR), and histochemical staining of CycB1;1::GUS supported the hypothesis that ectopic cell division occurred in the sef hypocotyls after germination. Both immunoblot and qRT-PCR results showed that WUS was ectopically expressed in sef hypocotyls. Luciferase activity, chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) showed that GLUTAMINE-RICH PROTEIN 23 (GRP23) expression can be activated by WUS and that GRP23 is a direct target gene of WUS. The phenotypes of 35S::GRP23 plants and GRP23 knockdown lines supported the notion that GRP23 mediates the effects of WUS on hypocotyl length. Together, our data suggest that ectopic expression of WUS in hypocotyl controls cell division through its target gene GRP23. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | WUSCHEL GENE ; SHOOT ; CYTOKININ ; PROTEIN ; GROWTH ; AUXIN ; CYCLE ; FATE ; GIBBERELLINS ; TOLERANCE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000325220000043 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Science Foundation of China for Innovative Research Groups(National Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27785]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dajian,Wang, Xiaomin,Wang, Min,et al. Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis[J]. PLOS ONE,2013,8(9). |
| APA | Zhang, Dajian.,Wang, Xiaomin.,Wang, Min.,Li, Junhua.,Guo, Xiaoyu.,...&Xu, Yunyuan.(2013).Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis.PLOS ONE,8(9). |
| MLA | Zhang, Dajian,et al."Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis".PLOS ONE 8.9(2013). |
入库方式： OAI收割
来源：植物研究所
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