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Genome-Wide Analysis of ZmDREB Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of Zea mays L.

文献类型：期刊论文

作者Liu, Shengxue6; Wang, Xianglan6; Wang, Hongwei6; Xin, Haibo; Yang, Xiaohong1; Yan, Jianbing7; Li, Jiansheng1; Lam-Son Phan Tran; Shinozaki, Kazuo3; Yamaguchi-Shinozaki, Kazuko
刊名PLOS GENETICS
出版日期2013
卷号9期号:9
ISSN号1553-7404
DOI10.1371/journal.pgen.1003790
文献子类Article
英文摘要The worldwide production of maize (Zea mays L.) is frequently impacted by water scarcity and as a result, increased drought tolerance is a priority target in maize breeding programs. While DREB transcription factors have been demonstrated to play a central role in desiccation tolerance, whether or not natural sequence variations in these genes are associated with the phenotypic variability of this trait is largely unknown. In the present study, eighteen ZmDREB genes present in the maize B73 genome were cloned and systematically analyzed to determine their phylogenetic relationship, synteny with rice, maize and sorghum genomes; pattern of drought-responsive gene expression, and protein transactivation activity. Importantly, the association between the nucleic acid variation of each ZmDREB gene with drought tolerance was evaluated using a diverse population of maize consisting of 368 varieties from tropical and temperate regions. A significant association between the genetic variation of ZmDREB2.7 and drought tolerance at seedling stage was identified. Further analysis found that the DNA polymorphisms in the promoter region of ZmDREB2.7, but not the protein coding region itself, was associated with different levels of drought tolerance among maize varieties, likely due to distinct patterns of gene expression in response to drought stress. In vitro, protein-DNA binding assay demonstrated that ZmDREB2.7 protein could specifically interact with the target DNA sequences. The transgenic Arabidopsis overexpressing ZmDREB2.7 displayed enhanced tolerance to drought stress. Moreover, a favorable allele of ZmDREB2.7, identified in the drought-tolerant maize varieties, was effective in imparting plant tolerance to drought stress. Based upon these findings, we conclude that natural variation in the promoter of ZmDREB2.7 contributes to maize drought tolerance, and that the gene and its favorable allele may be an important genetic resource for the genetic improvement of drought tolerance in maize.
学科主题Genetics & Heredity
出版地SAN FRANCISCO
WOS关键词ARABIDOPSIS TRANSCRIPTION FACTOR ; CIS-ACTING ELEMENT ; FUNCTIONAL-ANALYSIS ; STRESS TOLERANCE ; LOW-TEMPERATURE ; ABSCISIC-ACID ; FREEZING TOLERANCE ; PLANT DROUGHT ; HIGH-SALT ; EXPRESSION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000325076600049
出版者PUBLIC LIBRARY SCIENCE
资助机构National Hi-Tech Research and Development Program of China(National High Technology Research and Development Program of China) ; National Basic Research Program of China(National Basic Research Program of China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Chinese Academy of Sciences (CAS)(Chinese Academy of Sciences)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27787]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
3.RIKEN, Ctr Sustainable Resource Sci, Signaling Pathway Res Unit, Tsurumi Ku, Yokohama, Kanagawa, Japan
4.Univ Tokyo, Grad Sch Agr & Life Sci, Lab Plant Mol Physiol, Bunkyo Ku, Tokyo, Japan
5.RIKEN, Ctr Sustainable Resource Sci, Gene Discovery Res Grp, Tsurumi Ku, Yokohama, Kanagawa, Japan
6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
7.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Shengxue,Wang, Xianglan,Wang, Hongwei,et al. Genome-Wide Analysis of ZmDREB Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of Zea mays L.[J]. PLOS GENETICS,2013,9(9).
APA Liu, Shengxue.,Wang, Xianglan.,Wang, Hongwei.,Xin, Haibo.,Yang, Xiaohong.,...&Qin, Feng.(2013).Genome-Wide Analysis of ZmDREB Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of Zea mays L..PLOS GENETICS,9(9).
MLA Liu, Shengxue,et al."Genome-Wide Analysis of ZmDREB Genes and Their Association with Natural Variation in Drought Tolerance at Seedling Stage of Zea mays L.".PLOS GENETICS 9.9(2013).

入库方式： OAI收割

来源：植物研究所

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