RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis
文献类型：期刊论文
| 作者 | Gao, Bin; Fan, Lusheng1; Li, Xingxing; Yang, Huifang; Liu, Fengluan; Wang, Ling; Xi, Lin; Ma, Nan; Zhao, Liangjun |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:8 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0072914 |
| 文献子类 | Article |
| 英文摘要 | In vitro, a new protocol of plant regeneration in rose was achieved via protocorm-like bodies (PLBs) induced from the rootlike organs named rhizoids that developed from leaf explants. The development of rhizoids is a critical stage for efficient regeneration, which is triggered by exogenous auxin. However, the role of cytokinin in the control of organogenesis in rose is as yet uncharacterized. The aim of this study was to elucidate the molecular mechanism of cytokinin-modulated rhizoid formation in Rosa canina. Here, we found that cytokinin is a key regulator in the formation of rhizoids. Treatment with cytokinin reduced callus activity and significantly inhibited rhizoid formation in Rosa canina. We further isolated the full-length cDNA of a type-A response regulator gene of cytokinin signaling, RcRR1, from which the deduced amino acid sequence contained the conserved DDK motif. Gene expression analysis revealed that RcRR1 was differentially expressed during rhizoid formation and its expression level was rapidly up-regulated by cytokinin. In addition, the functionality of RcRR1 was tested in Arabidopsis. RcRR1 was found to be localized to the nucleus in GFP-RcRR1 transgenic plants and overexpression of RcRR1 resulted in increased primary root length and lateral root density. More importantly, RcRR1 overexpression transgenic plants also showed reduced sensitivity to cytokinin during root growth; auxin distribution and the expression of auxin efflux carriers PIN genes were altered in RcRR1 overexpression plants. Taken together, these results demonstrate that RcRR1 is a functional type-A response regulator which is involved in cytokinin-regulated rhizoid formation in Rosa canina. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | IN-VITRO PROPAGATION ; SIGNAL-TRANSDUCTION ; PLANT-REGENERATION ; LEAF EXPLANTS ; ARABIDOPSIS ; BIOSYNTHESIS ; EXPRESSION ; PROTEINS ; GROWTH ; SIZE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000323734600042 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27788]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.China Agr Univ, Dept Ornamental Hort & Landscape Architecture, Beijing 100094, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Bin,Fan, Lusheng,Li, Xingxing,et al. RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis[J]. PLOS ONE,2013,8(8). |
| APA | Gao, Bin.,Fan, Lusheng.,Li, Xingxing.,Yang, Huifang.,Liu, Fengluan.,...&Zhao, Liangjun.(2013).RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis.PLOS ONE,8(8). |
| MLA | Gao, Bin,et al."RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis".PLOS ONE 8.8(2013). |
入库方式： OAI收割
来源：植物研究所
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