A global profiling of uncapped mRNAs under cold stress reveals specific decay patterns and endonucleolytic cleavages in Brachypodium distachyon
文献类型：期刊论文
| 作者 | Zhang, Jingyu; Mao, Zhiwei; Chong, Kang |
| 刊名 | GENOME BIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 14期号:8 |
| 关键词 | mRNA stability decapping cold stress PARE Brachypodium distachyon |
| ISSN号 | 1474-760X |
| DOI | 10.1186/gb-2013-14-8-r92 |
| 文献子类 | Article |
| 英文摘要 | Background: mRNA degradation is a critical factor in determining mRNA abundance and enables rapid adjustment of gene expression in response to environmental stress. The involvement of processing bodies in stress response suggests a role for decapping-mediated mRNA degradation. However, little is known about the role of mRNA degradation under stressful environmental conditions. Results: Here, we perform a global study of uncapped mRNAs, via parallel analysis of RNA ends (PARE), under cold stress in Brachypodium distachyon. Enrichment analysis indicates that degradation products detected by PARE are mainly generated by the decapping pathway. Endonucleolytic cleavages are detected, uncovering another way of modulating gene expression. PARE and RNA-Seq analyses identify four types of mRNA decay patterns. Type II genes, for which light-harvesting processes are over-represented in gene ontology analyses, show unchanged transcript abundance and altered uncapped transcript abundance. Uncapping-mediated transcript stability of light harvesting-related genes changes significantly in response to cold stress, which may allow rapid adjustments in photosynthetic activity in response to cold stress. Transcript abundance and uncapped transcript abundance for type III genes changes in opposite directions in response to cold stress, indicating that uncapping-mediated mRNA degradation plays a role in regulating gene expression. Conclusion: To our knowledge, this is the first global analysis of mRNA degradation under environmental stress conditions in Brachypodium distachyon. We uncover specific degradation and endonucleolytic cleavage patterns under cold stress, which will deepen our understanding of mRNA degradation under stressful environmental conditions, as well as the cold stress response mechanism in monocots. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 出版地 | LONDON |
| WOS关键词 | LOW-TEMPERATURE ; P-BODIES ; GENE-EXPRESSION ; PARALLEL ANALYSIS ; PROTEIN-KINASE ; WIDE ANALYSIS ; IDENTIFICATION ; ARABIDOPSIS ; PHOTOSYNTHESIS ; MICRORNAS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000328195400010 |
| 出版者 | BMC |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences(Chinese Academy of Sciences) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27794]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jingyu,Mao, Zhiwei,Chong, Kang. A global profiling of uncapped mRNAs under cold stress reveals specific decay patterns and endonucleolytic cleavages in Brachypodium distachyon[J]. GENOME BIOLOGY,2013,14(8). |
| APA | Zhang, Jingyu,Mao, Zhiwei,&Chong, Kang.(2013).A global profiling of uncapped mRNAs under cold stress reveals specific decay patterns and endonucleolytic cleavages in Brachypodium distachyon.GENOME BIOLOGY,14(8). |
| MLA | Zhang, Jingyu,et al."A global profiling of uncapped mRNAs under cold stress reveals specific decay patterns and endonucleolytic cleavages in Brachypodium distachyon".GENOME BIOLOGY 14.8(2013). |
入库方式： OAI收割
来源：植物研究所
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