gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii
文献类型：期刊论文
| 作者 | Ling, Yu; Chen, Tong2; Jing, Yanping; Fan, Lusheng2; Wan, Yinglang; Lin, Jinxing2 |
| 刊名 | PLANTA
 |
| 出版日期 | 2013 |
| 卷号 | 238期号:5页码:831-843 |
| 关键词 | GABA Pollen tube Calcium Actin filaments Cell wall |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-013-1938-5 |
| 文献子类 | Article |
| 英文摘要 | gamma-Aminobutyric acid (GABA) is a four-carbon non-protein amino acid found in a wide range of organisms. Recently, GABA accumulation has been shown to play a role in the stress response and cell growth in angiosperms. However, the effect of GABA deficiency on pollen tube development remains unclear. Here, we demonstrated that specific concentrations of exogenous GABA stimulated pollen tube growth in Picea wilsonii, while an overdose suppressed pollen tube elongation. The germination percentage of pollen grains and morphological variations in pollen tubes responded in a dose-dependent manner to treatment with 3-mercaptopropionic acid (3-MP), a glutamate decarboxylase inhibitor, while the inhibitory effects could be recovered in calcium-containing medium supplemented with GABA. Using immunofluorescence labeling, we found that the actin cables were disorganized in 3-MP treated cells, followed by the transition of endo/exocytosis activating sites from the apex to the whole tube shank. In addition, variations in the deposition of cell wall components were detected upon labeling with JIM5, JIM7, and aniline blue. Our results demonstrated that calcium-dependent GABA signaling regulates pollen germination and polarized tube growth in P. wilsonii by affecting actin filament patterns, vesicle trafficking, and the configuration and distribution of cell wall components. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | CELL-WALL CONSTRUCTION ; TUBE GROWTH ; ARABIDOPSIS-THALIANA ; UP-REGULATION ; CYTOSKELETON ; MICROTUBULES ; EXPRESSION ; RECEPTORS ; BINDING ; ACCUMULATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000326073800003 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Key Basic Research Program from MOST ; Program of Introducing Talents of Discipline to Universities (111 project)(Ministry of Education, China - 111 Project) ; Major Science Foundation of Ministry of Education of China ; National Forestry Public Benefit Research Foundation |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27902]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, Yu,Chen, Tong,Jing, Yanping,et al. gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii[J]. PLANTA,2013,238(5):831-843. |
| APA | Ling, Yu,Chen, Tong,Jing, Yanping,Fan, Lusheng,Wan, Yinglang,&Lin, Jinxing.(2013).gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii.PLANTA,238(5),831-843. |
| MLA | Ling, Yu,et al."gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii".PLANTA 238.5(2013):831-843. |
入库方式： OAI收割
来源：植物研究所
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