The Arabidopsis exocyst subunit SEC3A is essential for embryo development and accumulates in transient puncta at the plasma membrane
文献类型：期刊论文
| 作者 | Zhang, Ying; Immink, Richard2; Liu, Chun-Ming ; Emons, Anne Mie; Ketelaar, Tijs
|
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2013 |
| 卷号 | 199期号:1页码:74-88 |
| 关键词 | Arabidopsis exocyst exocytosis green fluorescent protein localization plasma membrane SEC3 secretion |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.12236 |
| 文献子类 | Article |
| 英文摘要 | The exocyst is a protein complex that is essential for polarized secretion in mammals and fungi. Although the exocyst is essential for plant development, its precise function has not been elucidated. We studied the role of exocyst subunit SEC3A in plant development and its subcellular localization. T-DNA insertional mutants were identified and complemented with a SEC3A-green fluorescent protein (GFP) fusion construct. SEC3A-GFP localization was determined using confocal microscopy. sec3a mutants are defective in the globular to heart stage transition in embryogenesis. SEC3A-GFP has similar cell plate localization to the other plant exocyst subunits. In interphase cells, SEC3A-GFP localizes to the cytoplasm and to the plasma membrane, where it forms immobile, punctate structures with discrete lifetimes of 2-40s. These puncta are equally distributed over the cell surface of root epidermal cells and tip growing root hairs. The density of puncta does not decrease after growth termination of these cells, but decreases strongly when exocytosis is inhibited by treatment with brefeldin A. SEC3A does not appear to be involved in polarized secretion for cell expansion in tip growing root hairs. The landmark function performed by SEC3 in mammals and yeast is likely to be conserved in plants. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1469-8137 |
| WOS关键词 | PLANT-CELL GROWTH ; AUXIN TRANSPORT ; COMPLEX ; GENE ; EXOCYTOSIS ; EXPRESSION ; SECRETION ; PHOSPHOLIPIDS ; ORGANIZATION ; DEPOSITION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000319520200011 |
| 出版者 | WILEY |
| 资助机构 | Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; Wageningen University |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27916]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Wageningen Univ, Cell Biol Lab, NL-6708 PB Wageningen, Netherlands 3.Univ Wageningen & Res Ctr, Plant Res Int, NL-6708 PB Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Zhang, Ying,Immink, Richard,Liu, Chun-Ming,et al. The Arabidopsis exocyst subunit SEC3A is essential for embryo development and accumulates in transient puncta at the plasma membrane[J]. NEW PHYTOLOGIST,2013,199(1):74-88. |
| APA | Zhang, Ying,Immink, Richard,Liu, Chun-Ming,Emons, Anne Mie,&Ketelaar, Tijs.(2013).The Arabidopsis exocyst subunit SEC3A is essential for embryo development and accumulates in transient puncta at the plasma membrane.NEW PHYTOLOGIST,199(1),74-88. |
| MLA | Zhang, Ying,et al."The Arabidopsis exocyst subunit SEC3A is essential for embryo development and accumulates in transient puncta at the plasma membrane".NEW PHYTOLOGIST 199.1(2013):74-88. |
入库方式： OAI收割
来源：植物研究所
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