Oryza sativa actin-interacting protein1 is required for rice growth by promoting actin turnover
文献类型：期刊论文
| 作者 | Shi, Meng4; Xie, Yurong; Zheng, Yiyan4; Wang, Junmin3; Su, Yi1; Yang, Qiuying; Huang, Shanjin |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2013 |
| 卷号 | 73期号:5页码:747-760 |
| 关键词 | actin actin-binding protein ADF actin turnover AIP1 Oryza sativa |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.12065 |
| 文献子类 | Article |
| 英文摘要 | Rapid actin turnover is essential for numerous actin-based processes. However, how it is precisely regulated remains poorly understood. Actin-interacting protein1 (AIP1) has been shown to be an important factor by acting coordinately with actin-depolymerizing factor (ADF)/cofilin in promoting actin depolymerization, the rate-limiting factor in actin turnover. However, the molecular mechanism by which AIP1 promotes actin turnover remains largely unknown in plants. Here, we provide a demonstration that AIP1 promotes actin turnover, which is required for optimal growth of rice plants. Specific down-regulation of OsAIP1 increased the level of filamentous actin and reduced actin turnover, whereas over-expression of OsAIP1 induced fragmentation and depolymerization of actin filaments and enhanced actin turnover. In vitro biochemical characterization showed that, although OsAIP1 alone does not affect actin dynamics, it enhances ADF-mediated actin depolymerization. It also caps the filament barbed end in the presence of ADF, but the capping activity is not required for their coordinated action. Real-time visualization of single filament dynamics showed that OsAIP1 enhanced ADF-mediated severing and dissociation of pointed end subunits. Consistent with this, the filament severing frequency and subunit off-rate were enhanced in OsAIP1 over-expressors but decreased in RNAi protoplasts. Importantly, OsAIP1 acts coordinately with ADF and profilin to induce massive net actin depolymerization, indicating that AIP1 plays a major role in the turnover of actin, which is required to optimize F-actin levels in plants. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | FULL-LENGTH CDNAS ; ROOT HAIR-GROWTH ; II FORMIN FH5 ; FILAMENT DYNAMICS ; FUNCTIONAL-ANALYSIS ; CELL ELONGATION ; CAPPING PROTEIN ; BARBED ENDS ; ARABIDOPSIS ; COFILIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000315359600004 |
| 出版者 | WILEY |
| 资助机构 | Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27918]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China 2.Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Meng,Xie, Yurong,Zheng, Yiyan,et al. Oryza sativa actin-interacting protein1 is required for rice growth by promoting actin turnover[J]. PLANT JOURNAL,2013,73(5):747-760. |
| APA | Shi, Meng.,Xie, Yurong.,Zheng, Yiyan.,Wang, Junmin.,Su, Yi.,...&Huang, Shanjin.(2013).Oryza sativa actin-interacting protein1 is required for rice growth by promoting actin turnover.PLANT JOURNAL,73(5),747-760. |
| MLA | Shi, Meng,et al."Oryza sativa actin-interacting protein1 is required for rice growth by promoting actin turnover".PLANT JOURNAL 73.5(2013):747-760. |
入库方式： OAI收割
来源：植物研究所
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