Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization
文献类型：期刊论文
| 作者 | Zhao, Xin1,3; Yang, Ning1,3; Wang, Tai1
|
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 12期号:11页码:5058-5071 |
| 关键词 | comparative proteomics proteins pollen grains pollen tubes generative cells sperm cells |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr400291p |
| 文献子类 | Article |
| 英文摘要 | In flowering plants, two sperm cells (SCs) are generated from a generative cell (GC) in the developing pollen grain or growing pollen tube and are then delivered to the embryo sac to initiate double fertilization. SC development and function specialization involve the strict control of the protein (gene) expression program and coordination of diverse cellular processes. However, because methods for collecting a large amount of highly purified GCs and SCs for proteomic and transcriptomic studies from a plant are not available, molecular information about the program and the interconnections is lacking. Here, we describe a method for obtaining a large quantity of highly purified GCs and SCs from just-germinated lily pollen grains and growing pollen tubes for proteomic analysis. Our observation showed that SCs had less condensed chromatin and more vacuole-like structures than GCs and that mature SCs were arrested at the G2 phase. Comparison of SC and GC proteomes revealed 101 proteins differentially expressed in the two proteomes. These proteins are involved in diverse cellular and metabolic processes, with preferential involvement in metabolism, the cell cycle, signaling, the ubiquitin/proteasome pathway, and chromatin remodeling. Impressively, almost all proteins in SCF complex-mediated proteolysis and the cell cycle were up-regulated in SCs, whereas those in chromatin remodeling and stress response were down-regulated. Our data also reveal the coordination of SCF complex-mediated proteolysis, cell cycle progression, and DNA repair in SC development and function specialization. This study revealed for the first time a difference in protein profiles between GCs and SCs. |
| 学科主题 | Biochemical Research Methods |
| 出版地 | WASHINGTON |
| 电子版国际标准刊号 | 1535-3907 |
| WOS关键词 | MALE GAMETOPHYTE DEVELOPMENT ; MALE GAMETIC CELLS ; UBA PROTEIN DDI1 ; F-BOX PROTEIN ; ARABIDOPSIS-THALIANA ; CYCLE PROGRESSION ; LILIUM-LONGIFLORUM ; MATURE POLLEN ; DNA-DAMAGE ; SACCHAROMYCES-CEREVISIAE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000326615000030 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | Chinese Ministry of Science and Technology(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27983]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xin,Yang, Ning,Wang, Tai. Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(11):5058-5071. |
| APA | Zhao, Xin,Yang, Ning,&Wang, Tai.(2013).Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization.JOURNAL OF PROTEOME RESEARCH,12(11),5058-5071. |
| MLA | Zhao, Xin,et al."Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization".JOURNAL OF PROTEOME RESEARCH 12.11(2013):5058-5071. |
入库方式： OAI收割
来源：植物研究所
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