Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis
文献类型：期刊论文
| 作者 | Xiao, Jun2; Zhang, Hong2; Xing, Lijing; Xu, Shujuan2; Liu, Huanhuan2; Chong, Kang; Xu, Yunyuan |
| 刊名 | JOURNAL OF PLANT PHYSIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 170期号:4页码:444-451 |
| 关键词 | Flowering Histone modification FLC HAM1/2 |
| ISSN号 | 0176-1617 |
| DOI | 10.1016/j.jplph.2012.11.007 |
| 文献子类 | Article |
| 英文摘要 | Histone acetylation is an important posttranslational modification associated with gene activation. In Arabidopsis, two MYST histone acetyltransferases HAM1 and HAM2 work redundantly to acetylate histone H4 lysine 5 (H4K5ace) in vitro. The double mutant ham1/ham2 is lethal, which suggests the critical role of HAM1 and HAM2 in development. Here, we used an artificial microRNA (amiRNA) strategy in Arabidopsis to uncover a novel function of HAM1 and HAM2. The amiRNA-HAM1/2 transgenic plants showed early flowering and reduced fertility. In addition, they responded normally to photoperiod, gibberellic acid treatment, and vernalization. The expression of flowering-repressor FLOWERING LOCUS C (FLC) and its homologues, MADS-box Affecting Flowering genes 3/4 (MAF3/4), were decreased in amiRNA-HAM1/2 lines. HAM1 overexpression caused late flowering and elevated expression of PLC and MAF3/4. Mutation of PLC almost rescued the late flowering with HAM1 overexpression, which suggests that HAM1 regulation of flowering time depended on PLC. Global H4 acetylation was decreased in amiRNA-HAM1/2 lines, but increased in HAM1-OE lines, which further confirmed the acetyltransferase activity of HAM1 in vivo. Chromatin immunoprecipitation revealed that H4 hyperacetylation and H4K5ace at FLC and MAF3/4 were less abundant in amiRNA-HAM1/2 lines than the wild type, but were enriched in HAM1-OE lines. Thus, HAM1 and HAM2 may affect flowering time by epigenetic modification of PLC and MAF3/4 chromatins at H4K5 acetylation. (C) 2012 Elsevier GmbH. All rights reserved. |
| 学科主题 | Plant Sciences |
| 出版地 | MUNICH |
| 电子版国际标准刊号 | 1618-1328 |
| WOS关键词 | RNA-BINDING PROTEIN ; TRANSCRIPTIONAL ADAPTER ; LOCUS-C ; TIME ; GENE ; CHROMATIN ; ACETYLATION ; DIMETHYLATION ; VERNALIZATION ; ACTIVATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000316303900010 |
| 出版者 | ELSEVIER GMBH |
| 资助机构 | National Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Innovation Grant of the Chinese Academy of Sciences(Chinese Academy of Sciences) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28000]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Jun,Zhang, Hong,Xing, Lijing,et al. Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(4):444-451. |
| APA | Xiao, Jun.,Zhang, Hong.,Xing, Lijing.,Xu, Shujuan.,Liu, Huanhuan.,...&Xu, Yunyuan.(2013).Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis.JOURNAL OF PLANT PHYSIOLOGY,170(4),444-451. |
| MLA | Xiao, Jun,et al."Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis".JOURNAL OF PLANT PHYSIOLOGY 170.4(2013):444-451. |
入库方式： OAI收割
来源：植物研究所
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