Dynamic analysis of Arabidopsis AP2 sigma subunit reveals a key role in clathrin-mediated endocytosis and plant development
文献类型：期刊论文
| 作者 | Fan, Lusheng2; Hao, Huaiqing; Xue, Yiqun2; Zhang, Liang3; Song, Kai2; Ding, Zhaojun4; Botella, Miguel A.; Wang, Haiyang6; Lin, Jinxing |
| 刊名 | DEVELOPMENT
 |
| 出版日期 | 2013 |
| 卷号 | 140期号:18页码:3826-3837 |
| 关键词 | AP2 complex AP-2 Clathrin-mediated endocytosis FCCS PIN auxin transporter VA-TIRFM |
| ISSN号 | 0950-1991 |
| DOI | 10.1242/dev.095711 |
| 文献子类 | Article |
| 英文摘要 | Clathrin-mediated endocytosis, which depends on the AP2 complex, plays an essential role in many cellular and developmental processes in mammalian cells. However, the function of the AP2 complex in plants remains largely unexplored. Here, we show in Arabidopsis that the AP2 sigma subunit mutant (ap2 sigma) displays various developmental defects that are similar to those of mutants defective in auxin transport and/or signaling, including single, trumpet-shaped and triple cotyledons, impaired vascular pattern, reduced vegetative growth, defective silique development and drastically reduced fertility. We demonstrate that AP2 sigma is closely associated and physically interacts with the clathrin light chain (CLC) in vivo using fluorescence cross-correlation spectroscopy (FCCS), protein proximity analyses and co-immunoprecipitation assays. Using variable-angle total internal reflection fluorescence microscopy (VA-TIRFM), we show that AP2 sigma-mCherry spots colocalize with CLC-EGFP at the plasma membrane, and that AP2 s-mCherry fluorescence appears and disappears before CLC-EGFP fluorescence. The density and turnover rate of the CLC-EGFP spots are significantly reduced in the ap2 sigma mutant. The internalization and recycling of the endocytic tracer FM4-64 and the auxin efflux carrier protein PIN1 are also significantly reduced in the ap2 sigma mutant. Further, the polar localization of PIN1-GFP is significantly disrupted during embryogenesis in the ap2 sigma mutant. Taken together, our results support an essential role of AP2 s in the assembly of a functional AP2 complex in plants, which is required for clathrin-mediated endocytosis, polar auxin transport and plant growth regulation. |
| 学科主题 | Developmental Biology |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1477-9129 |
| WOS关键词 | REFLECTION FLUORESCENCE MICROSCOPY ; CORRELATION SPECTROSCOPY ; ADAPTER COMPLEX ; RECEPTOR ; BINDING ; PROTEINS ; AP-2 ; NETWORK ; GROWTH ; DOMAIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000323698100012 |
| 出版者 | COMPANY BIOLOGISTS LTD |
| 资助机构 | National Basic Research Program of China(National Basic Research Program of China) ; project Program for Creative Research Teams ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28019]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 5.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China 6.Botella, Miguel A.] Univ Malaga, Dept Biol Celular Genet & Fisiol, E-29071 Malaga, Spain |
| 推荐引用方式 GB/T 7714 | Fan, Lusheng,Hao, Huaiqing,Xue, Yiqun,et al. Dynamic analysis of Arabidopsis AP2 sigma subunit reveals a key role in clathrin-mediated endocytosis and plant development[J]. DEVELOPMENT,2013,140(18):3826-3837. |
| APA | Fan, Lusheng.,Hao, Huaiqing.,Xue, Yiqun.,Zhang, Liang.,Song, Kai.,...&Lin, Jinxing.(2013).Dynamic analysis of Arabidopsis AP2 sigma subunit reveals a key role in clathrin-mediated endocytosis and plant development.DEVELOPMENT,140(18),3826-3837. |
| MLA | Fan, Lusheng,et al."Dynamic analysis of Arabidopsis AP2 sigma subunit reveals a key role in clathrin-mediated endocytosis and plant development".DEVELOPMENT 140.18(2013):3826-3837. |
入库方式： OAI收割
来源：植物研究所
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