Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites
文献类型：期刊论文
| 作者 | Guo, Yan-Wu; Guo, Hui-Li; Li, Xing; Huang, Li-Li; Zhang, Bo-Ning; Pang, Xiao-Bin; Liu, Ben-Ye; Ma, Lan-Qing; Wang, Hong6 |
| 刊名 | PLANT BIOTECHNOLOGY REPORTS
 |
| 出版日期 | 2013 |
| 卷号 | 7期号:3页码:371-381 |
| 关键词 | Chalcone synthase Gene evolution Polygonum cuspidatum Resveratrol Stilbene synthase Type III polyketide synthase |
| ISSN号 | 1863-5466 |
| DOI | 10.1007/s11816-012-0271-y |
| 文献子类 | Article |
| 英文摘要 | In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum. |
| 学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 出版地 | NEW YORK |
| WOS关键词 | CHALCONE SYNTHASE ; AROMATIC POLYKETIDE ; ENZYMATIC FORMATION ; 3-INTRON GENE ; BIOSYNTHESIS ; FAMILY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000321896900017 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Key Natural Science Foundation of Beijing Municipality(Beijing Natural Science Foundation) ; Foundation of Beijing Municipal Education Committee ; Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28021]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Henan Univ, Inst Pharm, Kaifeng 475004, Peoples R China 2.Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China 3.China Agr Univ, Minist Agr PR China, Key Lab Urban Agr North, Beijing 102206, Peoples R China 4.[Guo, Hui-Li 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Huang, Li-Li 7.Li, Xing 8.[Guo, Yan-Wu 9.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 10.Beijing 2 Middle Sch, Beijing 100010, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,et al. Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites[J]. PLANT BIOTECHNOLOGY REPORTS,2013,7(3):371-381. |
| APA | Guo, Yan-Wu.,Guo, Hui-Li.,Li, Xing.,Huang, Li-Li.,Zhang, Bo-Ning.,...&Wang, Hong.(2013).Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites.PLANT BIOTECHNOLOGY REPORTS,7(3),371-381. |
| MLA | Guo, Yan-Wu,et al."Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites".PLANT BIOTECHNOLOGY REPORTS 7.3(2013):371-381. |
入库方式： OAI收割
来源：植物研究所
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