Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.
文献类型：期刊论文
| 作者 | Pu, Gao-Bin; Ma, Dong-Ming; Wang, Hong1; Ye, He-Chun; Liu, Ben-Ye |
| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:1页码:32-37 |
| 关键词 | Artemisia annua L. Glandular trichome Amorpha-4,11-diene synthase Artemisinin Immunocytochemistry Immunohistochemistry |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-012-0472-0 |
| 文献子类 | Article |
| 英文摘要 | Artemisia annua L. is the only commercial source of the widely used antimalarial compound artemisinin. Biosynthesis of artemisinin has been proposed to take place in glandular trichomes. The first committed step in the conversion of farnesyl diphosphate (FDP) to artemisinin is conducted by the amorpha-4,11-diene synthase (ADS). To explore the organ-specific and developmental distributions of ADS, rabbit polyclonal antibodies were raised against recombinant ADS produced in Escherichia coli from the corresponding A. annua cDNA. Protein gel blot analysis of different A. annua organs showed that ADS was most abundant in young leaves and flower buds. Minor amounts of ADS were found in mature leaves. These findings were generally consistent with the analysis of the transcript level of the ADS gene. Immunolocalization of ADS showed strong positive staining in apical meristems, young leaves and glandular trichomes. No staining was observed in other cells of the leaf. The whole mount hybridization revealed that ADS was not expressed in all glandular trichomes of mature leaves. Specific staining of ADS could be detected in about 10-40 % of glandular trichomes. |
| 学科主题 | Biochemical Research Methods ; Plant Sciences |
| 出版地 | NEW YORK |
| WOS关键词 | GLANDULAR TRICHOMES ; KEY ENZYME ; MOLECULAR-CLONING ; ESSENTIAL OIL ; BIOSYNTHESIS ; PLANTS ; TISSUES ; GENES ; HOP |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000313412900004 |
| 出版者 | SPRINGER |
| 资助机构 | National High-tech R&D Program (863) of the Ministry of Sciences and Technology, China(National High Technology Research and Development Program of China) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28039]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shandong Yingcai Univ, Jinan 250104, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Gao-Bin,Ma, Dong-Ming,Wang, Hong,et al. Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(1):32-37. |
| APA | Pu, Gao-Bin,Ma, Dong-Ming,Wang, Hong,Ye, He-Chun,&Liu, Ben-Ye.(2013).Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L..PLANT MOLECULAR BIOLOGY REPORTER,31(1),32-37. |
| MLA | Pu, Gao-Bin,et al."Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.".PLANT MOLECULAR BIOLOGY REPORTER 31.1(2013):32-37. |
入库方式： OAI收割
来源：植物研究所
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