Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco
文献类型：期刊论文
| 作者 | Zhang, Zhao; Fradin, Emilie; de Jonge, Ronnie; van Esse, H. Peter; Smit, Patrick; Liu, Chun-Ming ; Thomma, Bart P. H. J.
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| 刊名 | MOLECULAR PLANT-MICROBE INTERACTIONS
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| 出版日期 | 2013 |
| 卷号 | 26期号:2页码:182-190 |
| ISSN号 | 0894-0282 |
| DOI | 10.1094/MPMI-06-12-0161-R |
| 文献子类 | Article |
| 英文摘要 | Recognition of pathogen effectors by plant immune receptors often leads to the activation of a hypersensitive response (HR), which is a rapid and localized cell death of plant tissue surrounding the site at which recognition occurs. Due to its particular amenability to transient assays for functional genetics, tobacco is a model for immune signaling in the Solanaceae plant family. Here, we show that coexpression of the tomato (Solanum lycopersicum) immune receptor Ve1 and the corresponding Verticillium effector protein Ave1 leads to HR only in particular tobacco species. Whereas HR is obtained in Nicotiana tabacum, no such response is obtained in N. benthamiana. Furthermore, our analysis revealed an endogenous Ve1 ortholog in Nicotiana glutinosa, as expression of Ave1 in absence of Ve1 induced a HR, and N. glutinosa was found to be resistant against race 1 Verticillium dahliae. We furthermore report the establishment of virus-induced gene silencing in N. tabacum for functional analysis of Ve1 signaling. Collectively, our data show that N. tabacum can be used as a model plant to study Ve1-mediated immune signaling. |
| 学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| 出版地 | ST PAUL |
| 电子版国际标准刊号 | 1943-7706 |
| WOS关键词 | RECEPTOR-LIKE PROTEINS ; TRANSIENT EXPRESSION SYSTEM ; FUNCTIONAL-ANALYSIS ; DISEASE RESISTANCE ; NICOTIANA-BENTHAMIANA ; PLANT-PATHOGEN ; BINARY VECTORS ; ALBO-ATRUM ; TOMATO ; VERSATILE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000314003000004 |
| 出版者 | AMER PHYTOPATHOLOGICAL SOC |
| 资助机构 | Netherlands Organization for Scientific Research (NWO-ALW)(Netherlands Organization for Scientific Research (NWO)) ; Wageningen University ; Centre for BioSystems Genomics (CBSG) ; ERA-NET Plant Genomics |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28117]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.[Fradin, Emilie 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Smit, Patrick 4.Thomma, Bart P. H. J.] Wageningen Univ, Phytopathol Lab, NL-6708 PB Wageningen, Netherlands 5.de Jonge, Ronnie 6.van Esse, H. Peter 7.Fradin, Emilie 8.[Zhang, Zhao 9.Thomma, Bart P. H. J.] Ctr BioSyst Genom, NL-6700 AB Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Zhang, Zhao,Fradin, Emilie,de Jonge, Ronnie,et al. Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2013,26(2):182-190. |
| APA | Zhang, Zhao.,Fradin, Emilie.,de Jonge, Ronnie.,van Esse, H. Peter.,Smit, Patrick.,...&Thomma, Bart P. H. J..(2013).Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco.MOLECULAR PLANT-MICROBE INTERACTIONS,26(2),182-190. |
| MLA | Zhang, Zhao,et al."Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco".MOLECULAR PLANT-MICROBE INTERACTIONS 26.2(2013):182-190. |
入库方式： OAI收割
来源：植物研究所
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