Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars
文献类型：期刊论文
| 作者 | Qu, Xiaolu; Zhang, Hua; Xie, Yurong; Wang, Juan; Chen, Naizhi; Huang, Shanjin1 |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2013 |
| 卷号 | 25期号:5页码:1803-1817 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.113.110940 |
| 文献子类 | Article |
| 英文摘要 | Apical actin filaments are crucial for pollen tube tip growth. However, the specific dynamic changes and regulatory mechanisms associated with actin filaments in the apical region remain largely unknown. Here, we have investigated the quantitative dynamic parameters that underlie actin filament growth and disappearance in the apical regions of pollen tubes and identified villin as the major player that drives rapid turnover of actin filaments in this region. Downregulation of Arabidopsis thaliana VILLIN2 (VLN2) and VLN5 led to accumulation of actin filaments at the pollen tube apex. Careful analysis of single filament dynamics showed that the severing frequency significantly decreased, and the lifetime significantly increased in vln2 vln5 pollen tubes. These results indicate that villin-mediated severing is critical for turnover and departure of actin filaments originating in the apical region. Consequently, the construction of actin collars was affected in vln2 vln5 pollen tubes. In addition to the decrease in severing frequency, actin filaments also became wavy and buckled in the apical cytoplasm of vln2 vln5 pollen tubes. These results suggest that villin confers rigidity upon actin filaments. Furthermore, an observed decrease in skewness of actin filaments in the subapical region of vln2 vln5 pollen tubes suggests that villin-mediated bundling activity may also play a role in the construction of actin collars. Thus, our data suggest that villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 出版地 | ROCKVILLE |
| WOS关键词 | F-ACTIN ; PHYSCOMITRELLA-PATENS ; DEPOLYMERIZING FACTOR ; POLARIZED GROWTH ; CELL-GROWTH ; EXTRACELLULAR CALCIUM ; INTERACTING PROTEIN1 ; DYNAMIC-BEHAVIOR ; BUNDLING PROTEIN ; CORTICAL ARRAY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000321035800025 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28118]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Xiaolu,Zhang, Hua,Xie, Yurong,et al. Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars[J]. PLANT CELL,2013,25(5):1803-1817. |
| APA | Qu, Xiaolu,Zhang, Hua,Xie, Yurong,Wang, Juan,Chen, Naizhi,&Huang, Shanjin.(2013).Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars.PLANT CELL,25(5),1803-1817. |
| MLA | Qu, Xiaolu,et al."Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars".PLANT CELL 25.5(2013):1803-1817. |
入库方式： OAI收割
来源：植物研究所
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